Analysis of Tissue Expression of Potential Target Genes of Bovine miR-6523a

doi:10.7668/hbnxb.20194400

Abstract

Abstract:

bta-miR-6523a is one of the genes related to bovine fat deposition screened by the previous research Shi Yuangang's group through sequencing data.In order to screen the target gene of bta-miR-6523a,identify its expression rule in different tissues,and explore the possible regulatory mechanism of bta-miR-6523a on bovine fat deposition,this study used a variety of database information to predict and screen the target gene of bta-miR-6523a,and enriched and analyzed the predicted functional pathway of the target gene.Finally,the expression of bta-miR-6523a in some tissues of adult cattle and calves was detected by qRT-PCR.The results showed that the predicted target genes of bta-miR-6523a were SCD,CIDEA,LIPE,etc.These predicted target genes were significantly enriched in Ras,Rap1,actin cytoskeleton regulation,Hippo and other signaling pathways related to adipocyte generation and differentiation,cytoskeleton organization and maintenance,and metabolic pathways.qRT-PCR results showed that the expression level of bovine miR-6523a was the highest in lung and the lowest in perirenal fat.The expression of bta-miR-6523a in heart and liver was significantly higher than that in subcutaneous fat,intermuscular fat,longissimus dorsi muscle and perirenal fat,but there was no significant difference among subcutaneous fat,intermuscular fat,longissimus dorsi muscle and perirenal fat.The expression of miR-6523a in subcutaneous fat,intermuscular fat and longissimus dorsi muscle of calves was significantly different,and significantly lower than that in adult bovine tissues.The high expression of bta-miR-6523a in lung tissue and lower expression in adipose tissue suggest that the biological effects of bta-miR-6523a and its target genes may be extensive.

Key words: Cattle, bta-miR-6523a, Target gene prediction, Functional enrichment

QU Chang, ZHANG Yanfeng, DING Yanling, SHI Yuangang, KANG Xiaolong. Analysis of Tissue Expression of Potential Target Genes of Bovine miR-6523a[J]. Acta Agriculturae Boreali-Sinica, 2024, 39(S1): 298-304. doi: 10.7668/hbnxb.20194400.

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Figures/Tables 9

References 35

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数据库名称 Database name	功能 Function	网址 Website
miRBase	miRNA序列数据库	http://www.mirbase.org/index.shtml/
TargetScan	miRNA靶基因预测	http://www.targetscan.org/
RNAhybrid	miRNA靶基因预测	http://bibiserv.techfak.uni-bielefeld.de/rnahybrid/
miRanda	miRNA靶基因预测	http://www.microRNA.org/
Veney	维恩图绘制	http://bioinfogp.cnb.csic.es/tools/Venny/index.html

数据库名称 Database name	功能 Function	网址 Website
miRBase	miRNA序列数据库	http://www.mirbase.org/index.shtml/
TargetScan	miRNA靶基因预测	http://www.targetscan.org/
RNAhybrid	miRNA靶基因预测	http://bibiserv.techfak.uni-bielefeld.de/rnahybrid/
miRanda	miRNA靶基因预测	http://www.microRNA.org/
Veney	维恩图绘制	http://bioinfogp.cnb.csic.es/tools/Venny/index.html

基因 Gene	引物序列(5'-3') Primer sequence(5'-3')	引物长度/bp Primer length
bta-miR-6523a	RTprimer:GTCGTATCCAGTGCAGGGTCCGAGG	50
	TATTCGCACTGGATACGACCCCTGC
	F:AGCTGGACTCTGGGGTAACTTTG	23
	R:ATCCAGTGCAGGGTCCGAGG	20
18S RNA	F:GTGGTGTTGAGGAAAGCAGACA	22
	R: TGATCACACGTTCCACCTCATC	22

基因 Gene	引物序列(5'-3') Primer sequence(5'-3')	引物长度/bp Primer length
bta-miR-6523a	RTprimer:GTCGTATCCAGTGCAGGGTCCGAGG	50
	TATTCGCACTGGATACGACCCCTGC
	F:AGCTGGACTCTGGGGTAACTTTG	23
	R:ATCCAGTGCAGGGTCCGAGG	20
18S RNA	F:GTGGTGTTGAGGAAAGCAGACA	22
	R: TGATCACACGTTCCACCTCATC	22

数据库来源 Database source	数目 Count	主要靶基因列表 List of main target genes
miRanda	1 639	SCD、LEP、PPARG等
RNAhybrid	4 584	SCD、CIDEC、CIDEA等
TargetScan	2 693	SCD、LIPE、PPARG等
Intersection of Sets	870	SCD、CIDEA、CIDEC、LIPE等

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