Abstract:

To clarify the coding sequence and molecular characteristics of the CDS1 gene in Qinchuan cattle and its expression characteristics in different tissues.The full coding region sequence of CDS1 gene was cloned by PCR amplification technique in Qinchuan cattle,and the sequence characteristics of its full coding region were analyzed by bioinformatics.qRT-PCR was used to detect the expression levels of CDS1 gene in brain,testis,pancreas,lung,spleen,kidney,rumen,longest dorsal muscle,liver,intramuscular fat and heart tissues using GAPDH as an internal reference gene.The results showed that the coding region of CDS1 gene in Qinchuan cattle was 1 392 bp,encoding 464 amino acids,and the CDS1 gene was highly conserved among different species and had the highest homology with rumen cattle;the protein encoded by CDS1 gene was a hydrophobic unstable transmembrane protein,mainly composed of α-helix and irregular convolutions,and the subcellular localization analysis showed that CDS1 protein was mainly distributed with endoplasmic reticulum and mitochondria.Protein interaction analysis showed that it interacted with PGS1,PPAPDC1 series,CDIPT,PLD series,CRLS1 and other riboproteins related to phospholipid synthesis,suggesting that CDS1 gene was involved in regulating phospholipid anabolic process;tissue expression analysis showed that CDS1 gene was widely expressed in various tissues,and the highest expression was in testis,and the expression level in brain was also higher.The tissue expression analysis showed that CDS1 gene was widely expressed in all tissues,and its expression level was highest in testis and higher in brain,both of which were significantly higher than that of other tissues,and the expression level was lowest in heart.

Key words: Qinchuan cattle, CDS1 genes, Cloning, Bioinformatics analysis, Tissue expression