Screening Differential Genes Related to Hypoxia Adaptation in Cattle by Using Transcriptome Analysis

doi:10.7668/hbnxb.20190727

Abstract

Abstract: To identify differentially expressed genes related to hypoxia adaptation in Tibetan cattle and analyze differentially expressed gene functions and pathways,the total RNA was extracted from the heart tissues, then sequenced by Illumina HiSeqTM 4000. The differentially expressed transcripts were screened by bioinformatics analysis, GO and KEGG enrichment analysis were used to study the function of differentially expressed genes. The accuracy of RNA-Seq results was verified by RT-qPCR. The results showed that there were 608 significant differentially expressed genes in Tibetan cattle compared with Sanjiang cattle, including 467 up genes and 141 down genes. The B2M,COX7C,NFATC1,ECE1,CAST genes with high expression levels were inferred hypoxia-adapted related genes. Differential transcripts of GO function enrichment analysis revealed that molecular functions, cell components, and biological processes were enriched to 327 entries, 253 entries, and 2 191 entries, of which the most enriched entries were cell development process, binding and transcription factor activity, cell leading edge. KEGG analysis showed that the differential transcripts were significantly enriched in eight pathways, screening for the hypoxia-adaptive candidate gene NFATC1 was involved in the cGMP-PKG pathway and the T cell receptor signaling pathway, and was predicted to participate in hypoxia-adapted regulation through the cGMP-PKG pathway and the T cell receptor signaling pathway. RNA-seq technology was used to analyze the transcriptome of Tibetan cattle and Sanjiang cattle heart tissue, and a large number of differentially expressed genes were obtained, which laid a foundation for studying the hypoxia adaptation mechanism of animals.

Key words: Cattle, Heart tissue, Hypoxia adaptation, Differentially expressed mRNA, Functional enrichment analysis

CLC Number:

S823.8

HOU Mengdian, WANG Hui, ZHONG Jincheng, CHAI Zhixin, YIXI Kangzhu, WANG Jikun, WANG Jiabo. Screening Differential Genes Related to Hypoxia Adaptation in Cattle by Using Transcriptome Analysis[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2020, 35(2): 217-227. doi: 10.7668/hbnxb.20190727.

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