Function of MYL3 Gene in Muscle Growth of Cattle

doi:10.7668/hbnxb.201750982

Abstract

Abstract: The aim of this study was to investigate the effects of myosin light chain 3 (MLC1v or MYL3) on the regulation of skeletal muscle transcription, and to identify its functional relationship with muscle growth and development. MYL3 gene was cloned and its biological structure was analyzed by bioinformatics method. The expression of MYL3 gene in different tissues and at different stages was studied by means of fluorescence quantitative PCR technique. Immunohistochemistry showed that the expression locus of MYL3 gene were in the dorsal longissimus muscle. Western Blotting was used to study the expression of MYL3 in different tissues and at different stages. The results showed that the MYL3 gene of Black cattle (sequence number:NM_001076501.2) was cloned with a full-length reading frame of 600 bp, encoding 199 amino acids. Phylogenetic tree analysis showed that its homology was higher. Gene expression analysis showed the highest expression in the heart, followed by the longissimus muscle, and little in other tissues. The expression of MYL3 gene increased with the increase of age. The MYL3 protein mainly expressed in the myofilament of skeletal myofibrils, and increased with the age of the month at different stages. Presumably MYL3 might play an important role in skeletal muscle growth and development.

Key words: MYL3, Black cattle, Luxi cattle, Longissimus muscle

CLC Number:

S823
Q78

LIU Ruili, WU Lei, YUAN Wei, BAI Xuejin, LÜ Juanjuan, DONG Yajuan. Function of MYL3 Gene in Muscle Growth of Cattle[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2019, 34(2): 221-228. doi: 10.7668/hbnxb.201750982.

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References

[1] Chang K C, Beuzen N D, Hall A D.Identification of microsatellites in expressed muscle genes:Assessment of a desmin (CT) dinucleotide repeat as a marker for meat quality[J].Veterinary Journal,2003,165(2):157-163.doi:10.1016/S1090-0233(02)00159-4.
[2] Andersen S P, Hedley P L, Page S P, Syrris P, Moolman-Smook J C, McKenna W J, Elliott P M, Christiansen M.A novel myosin essential light chain mutation causes hypertrophic cardiomyopathy with late onset and low expressivity[J]. Biochemistry Research International, 2012(6):685108.doi:10.1155/2012/685108.
[3] Zhang C L, Wang J M, Wang G Z, Ji Z A, Liu Z H, Chao T L.Molecular cloning and mRNA expression analysis of sheep MYL3 and MYL4 genes[J].Gene,2016,577(2):209-214.doi:10.1016/j.gene.2015.11.041.
[4] Meder B L C, Rottbauer C.A single serine in the carboxyl terminus of cardiac essential myosin light chain-1 controls cardiomyocyte contractility in vivo[J].Circulation Research,2009,104(5):650-659.doi:10.1161/CIRCRESAHA.108.186676.
[5] Jay A, Chikarmane R, Poulik J, Misra V K.Infantile hypertrophic cardiomyopathy associated with a novel MYL3 mutation[J].Cardiology,2013,124(4):248-251.doi:10.1159/000347138.
[6] Gómez J, Reguero J R, Morís C, Martín M, Alvarez V, Alonso B, Iglesias S, Cotoí E.Mutation analysis of the main hypertrophic cardiomyopathy genes using multiplex amplification and semiconductor next-generation sequencing[J].Circulation Journal:Official Journal of the Japanese Circulation Society,2014,78(12):2963-2971.doi:10.1253/circj.cj-14-0628.
[7] Komurcu-Bayrak E, Ozsait B, Erginel-Unaltuna N.Isolation and analysis of genes mainly expressed in adult mouse heart using subtractive hybridization cDNA library[J].Molecular Biology Reports,2012,39(8):8065-8074.doi:10.1007/s11033-012-1653-5.
[8] Koning M, Werker P M.VanLuyn M J,Harmsen M C.Hypoxia promotes proliferation of humanmyogenic satellite cells:A potential benefactor in tissue engineering of skeletal muscle[J].Tissue Engineering Part A,2010,17(13):1747-1758.doi:10.1089/ten.tea.2010.0624.
[9] 杨莉, 董雅娟, 柏学进, 赵仕全, 牛召珊, 张新浩. 布莱凯特黑牛PDSS1 基因的克隆测序及生物信息学分析[J]. 中国畜牧杂志, 2013, 49(21):14-18.doi:10.3969/j.issn.0258-7033.2013.21.004.
Yang L, Dong Y J, Bai X J, Zhao S Q, Niu Z S, Zhang X H. Cloning,sequencing and bioinformatics analysis of PDSS1 gene in black cattle black cattle[J]. Chinese Journal of Animal Science, 2013,49(21):14-18.
[10] 宫本芝, 楚惠民, 王富国. 鲁西黄牛品种资源综述[J]. 中国牛业科学, 2015, 41(2):65-67.doi:10.3969/j.issn.1001-9111.2015.02.019.
Gong B Z, Chu H M, Wang F G. A summary of the resources of Luxi cattle[J]. China Cattle Science, 2015,41(2):65-67.
[11] 刘瑞莉, 康晓晨, 吴磊, 袁玮, 董雅娟. MYLPF 基因在肉牛肌肉生长过程中的功能研究[J]. 中国畜牧兽医, 2017, 44(11):3236-3242.doi:CNKI:SUN:GWXK.0.2017-11-018.
Liu R L, Kang X C, Wu L, Yuan W, Dong Y J. Functional study of MYLPF gene in muscle growth of beef cattle[J]. Chinese Journal of Animal Husbandry and Veterinary Medicine, 2017,44(11):3236-3242.
[12] Shi J, Robert T Kennedy.New developments in Western Blot technology[J].Chinese Chemical Letters,2015,26(4):416-418.doi:10.1016/j.cclet.2015.01.021.
[13] 刘瑞莉, 吴磊, 袁玮, 董雅娟. MYL2 基因在肌肉生长过程中的研究[J]. 黑龙江畜牧兽医, 2018(3):10-14, 252.doi:10.13881/j.cnki.hljxmsy.2017.07.0338.
Liu R L, Wu L, Yuan W, Dong Y J. Study on MYL2 gene in muscle growth process[J]. Heilongjiang Animal Science and Veterinary Medicine, 2018(3):10-14, 252.
[14] Ning L. Animal genetics(Pt 3)[M].Beijing:China Agriculture Press,2011.
[15] 张春兰. 小尾寒羊和杜泊羊臂二头肌转录组及肌球蛋白轻链基因家族结构特征分析[D]. 泰安:山东农业大学, 2014:46-47.
Zhang C L. Studies on bimodular transcriptome and myosin light chain gene family structure of small tail Han sheep and dorper[D].Tyan:Shandong Agricultural University, 2014:46-47.
[16] 李宁. 动物遗传学[M]. 3版. 北京:中国农业出版社, 2011.
Li N. Animal genetics[M]. 3rd ed. Beijing:China Agriculture Press,2011.
[17] Sherwood J G, Lowey S.A point mutation in the regulatory light chain reduces the step size of skeletal muscle myosin[J].PNAS,2004,101(30):10973-10978.doi:10.1073/pnas.0401699101.
[18] Tang Z, Li Y, Wan P, Li X P, Zhao S H, Liu B, Fan B, Zhu M J, Yu M, Li K.Long SAGE analysis of skeletal muscle at three prenatal stages in Tongcheng and Landrace pigs[J].Genome Biology,2007,8(6):R115.doi:10.1186/gb-2007-8-6-r115.
[19] 顾志良, 邵芳, 石亦静, 吴小娟. 鹅MYL1 基因的克隆及胚胎期表达特征分析[J]. 常熟理工学院学报, 2014, 28(2):7-12.doi:10.3969/j.issn.1008-2794.2014.02.002.
Gu Z L, Shao F, Dan Y J, Wu X J. Cloning and characterization of embryo MYL1 gene[J]. Journal of Changshu Institute of Technology, 2014,28(2):7-12.
[20] 谢遇春. 内蒙古绒山羊骨骼肌差异蛋白质组学研究[D]. 呼和浩特:内蒙古农业大学, 2018.
Xie Y C. Differential proteomics study on skeletal muscle of Inner Mongolia cashmere goat[D].Hohhut:Inner Mongolia Agricultural University, 2018.
[21] Ryan M T,O'halloran A M,hamill R M,Davey G C,Gil M,Southwood O I,Sweeney T.Polymorphisms in the regulatory region of the porcine MYLPF gene arerelated to meat quality traits in the large white breed[J]. Meat Science,2016(113):104-106.doi:10.1016/j.meatsci.2015.11.016.
[22] 王志秀. 基于转录组和蛋白组数据鉴定猪肌肉生长和脂肪沉积相关的基因[D]. 北京:中国农业大学, 2017.
Wang Z X. Identification of genes related to muscle growth and fat deposition in pigs based on transcriptome and proteomdata[D].Beijing:China Agricultural University, 2017.
[23] Lee A, Suh Y, Wick M P, Lee K.Temporal myosin heavy chain isoform expression transitions faster in broiler chickens compared with Single Comb White Leghorns[J].Poultry Science,2012,91(11):2872-2876.doi:10.3382/ps.2012-02232.
[24] Ling F, Fang W, Chen Y S, Li J Q, Liu X H, Wang L L, Zhang H, Chen S L, Mei Y J, Du H L, Wang C.Identification of novel transcripts from the porcine MYL1 gene and initial characterization of its promoters[J].Molecular and Cellular Biochemistry,2010,343(1/2):239-247.doi:10.1007/s11010-010-0519-1.

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