Abstract: The aim of this study was to screen the differential genes of skeletal muscle based on transcriptome,to explore the effect of different genes on the regulation of skeletal muscle transcription,and to identify the functional relationship with muscle growth and development,providing a theoretical basis for studying the molecular mechanism of skeletal muscle growth and development. Twelve months old Black cattle and Luxi cattle dorsal longissimus muscle were harvested,sequenced by Illumina 2500 and sequenced by comprehensive bioinformatics analysis (differential gene screening,GO classification,KEGG enrichment analysis and protein interaction Network,etc.) to screen for the regulation genes of skeletal muscle differentiation. There were 13 242 and 13 350 genes in Black cattle and Luxi cattle,respectively,for a total of 13 758 genes. The expression level of 5 500 genes was the same,accounting for 39.98% of the total reference gene,while the expression level of 8 258 genes was different,accounting for 60.02%. 554 differentially expressed genes were obtained as differentially expressed genes. Among them,296 genes were up-regulated and 258 genes were down-regulated. GO functional annotation results showed that there were 22 641,7 720 and 4 414 genes in biological processes,cell components and molecular functions,respectively. Of these,29 genes were involved in the development and differentiation of muscle cells; KEGG was enriched in Wnt,CMC and TGF-β,DCM,ROAC,VSMC six muscle growth and development related pathways,screening skeletal muscle gene MYL2,MYL3 and MYLPF. MYL2, MYL3 and MYLPF is a member of Wnt,DMC and TGF-β signaling pathways involved in the formation of muscle cells and muscle tissue,presumably MYL2, MYL3 and MYLPF may play an important role in skeletal muscle growth and development.

Key words: Black cattle, Luxi cattle, Transcriptome, Skeletal muscle

CLC Number: 

• S823