The Influence of Bemisia tabaci MED on Detoxification and Protective Enzymes after Feeding on ToCV-infected Tomato

doi:10.7668/hbnxb.20192207

Abstract

Abstract: To clarify the defense responses of Bemisia tabaci after feeding on ToCV-infected tomato, RNA-Seq analysis and qRT-PCR were performed to explore changes that occurred with the internal detoxification and protective enzyme systems as well as with the gene expression and metabolic functions 6, 12, 24, 48 hours respectively after B. tabaci MED fed on both ToCV-infected and uninfected tomato plants. The results showed that the transcription level of detoxification enzymes P450, GST, CarE and protective enzymes SOD, POD, CAT made different changes when B. tabaci MED feeding on ToCV-infected tomato at different times. The genes expression levels of P450, GST, CarE, SOD and POD tended to be up-regulated with the exception of CAT, among which P450 and SOD played the most major roles. Through GO annotation and KEGG function analysis of DEGs, it was found that biological function and metabolic pathways of DEGs of B. tabaci MED after feeding on ToCV-infected tomato for 12 h were different from other time points. After feeding for 12 h, B. tabaci MED mainly decomposed protein through lysosomes, while biosynthesis of amino acids to proteins were carried out through ribosomes at other times. In conclusion, B. tabaci that feeding on ToCV-infected tomato might have caused changes on the gene expression of detoxification and protective enzymes, and regulated the autoimmune response to ToCV invasion through lysosomal pathway. Among them, 12 hours feeding period might be the critical point for B. tabaci to interact with ToCV. The results here are helpful to further clarify the defense mechanism of B. tabaci toward ToCV infections, enrich the interaction theory of B. tabaci -ToCV-host, and provide a scientific basis for effective control of ToCV in the field.

Key words: Bemisia tabaci MED, Transcriptome, Tomato chlorosis virus, Detoxification enzymes, Protective enzymes

CLC Number:

S436.4

DAI Huijie, CHENG Lin, CAO Hui, ZHU Xiaoping, ZHAO Jing. The Influence of Bemisia tabaci MED on Detoxification and Protective Enzymes after Feeding on ToCV-infected Tomato[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2021, 36(4): 172-183. doi: 10.7668/hbnxb.20192207.

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