Abscisic Acid Metabolism and Differential Gene Expression Response to Water Deficit in the Oil Sunflower

doi:10.7668/hbnxb.20192019

Abstract

Abstract: In order to understand the differential gene mining and molecular regulation mechanism of abscisic acid metabolism of oil sunflower in response to drought stress, high-throughput transcriptome sequencing and physiological characteristics were measured on oil sunflower under normal water supply (CK) and drought stress (T) treatments. The results showed that the content of abscisic acid (ABA) was the highest at 8 d of drought stress, at 23.55 ng/mL, and higher than 0 d at 4,8 d of drought stress. The differentially expressed genes were screened and compared with CK on 8 and 16 days of drought stress, the number of differentially expressed genes in the treatment group was 940, 1 743, and the number of genes that were significantly down-regulated was 1 752, 3 037, respectively. According to the functional annotations of KEGG and GO, there were more gene types under drought stress at 16 d than at 8 d. Among them, the most involved biological processes, the second was involved in cell components, and the least involved in molecular functions. The main metabolic pathways annotated by KEGG were plant MAPK signal transduction pathway, plant hormone signal transduction, glycerophospholipid metabolism and so on. Analyzed the hormone-related genes under drought stress for 8 days. A total of 39 differentially expressed genes were found, and 7 genes were related to abscisic acid metabolism. The transcription factors involved in abscisic acid metabolism were ABF2, SAPK2, PP2C, AHG1, PYL2 and SAPK3 were up-regulated, and the expression level of drought stress was higher than that of CK. The conclusion of this study was that 7 different genes related to the up-regulation of ABA metabolism in oil sunflower under drought stress were discovered. The transcription factors involved in the metabolism of abscisic acid (PYR/PYL→PP2C→SnRK2→ABF) were all up-regulated, indicating that the plant when suffering from drought stress, it would resist drought stress by accumulating hormones such as abscisic acid.

Key words: Oil sunflower, Drought stress, Transcriptome sequencing, Hormone metabolism, Abscisic acid

CLC Number:

Q78
S565.03

ZHAO Yajie, ZHAO Xuanwei, TIAN Zhendong, HU Shuping, BAO Haizhu. Abscisic Acid Metabolism and Differential Gene Expression Response to Water Deficit in the Oil Sunflower[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2021, 36(6): 54-62. doi: 10.7668/hbnxb.20192019.

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