Abstract: Genetics analysis and fine mapping of osles3,a mutant with early senescent leaf in rice,were conducted to explore the molecular mechanism of rice leaf premature senescence.A leaf early senescence mutant osles3 was obtained from a natural mutant library of the Japonica variety Zhonghua 11.Phenotype and agronomic traits of osles3 and its wild-type were identified.The F₂ population from mutant crossed to ZS97(Indica cultivar) was used to mapping the gene OsLES3 by the map cloning method,and the structure and function of OsLES3 were analyzed by bioinformatics method.Phenotypic observation showed that the mutant began to have water-soaked senescence spots in the late tillering period,and the whole plant senescence at maturity. osles3 mutant showed extremely significantly decreased in plant height,tiller number,seed setting rate,grain number per panicle and 1000-grain weight compared to the wild-type plant.Genetic analysis revealed that the mutant phenotype was controlled by a pair of nuclear recessive gene on the long arm of chromosome 3,between two markers RM15524 and RM15551 with 0.9,0.5 cM genetic distance,respectively.Morever,sequence polymorphorism and bioinformatic analysis revealed that 988 bp fragment insertion and 54 bp fragment deletion in 5' Čupstream of translation initiation ATG of OsLES3,which encoded a LSD1 zinc finger domain containing protein.This research completed the fine mapping of the rice premature leaf senescence mutant osles3,and revealed that the mutant phenotype might be caused by some DNA fragment deletion and insertion in OsLES3 gene.

Key words: Rice, Early senescence mutant, Gene mapping, Bioinformatic

CLC Number: 

• Q78

• S511.03