Cloning and Function Analysis of TaJIP2 Gene Negatively Regulating Growth, Maturity and Yield in Triticum aestivum L.

doi:10.7668/hbnxb.20192370

Abstract

Abstract: To explore the regulation mechanism of Jasmonic acid(JA) on growth,maturation and yield in wheat,the gene TaJIP2 was cloned from wheat Bainong 207 by homologous cloning method.Analysis of tissue differential expression showed that TaJIP2 gene was expressed in all tissues and the expression level was the highest in wheat hull.The TaJIP2 gene RNA interference(RNAi) vector was constructed to conduct genetic transformation and functional analysis in wheat.The results showed that silencing TaJIP2 gene increased the key enzymes activities of jasmonic acid biosynthesis,allene oxide cyclase(AOC) and 12-oxo-phytodienoic acid reductase(OPR),and JA content,and the difference in JA content was mainly reflected in the roots.At the same time,it significantly promoted the growth of roots and above ground parts in wheat,and the difference was also mainly in root growth.In addition,observation of the ultrastructure of root tip cells showed that the root tip cells of TaJIP2 gene silenced plants were complete in structure,rich in variety and number of organelles,and strong in cell activity,thus promoting root growth.Silencing TaJIP2 significantly improved the photosynthetic efficiency of wheat leaves,made the heading time earlier,and significantly increased the grain length and width,spike number per plant,grain number per spike,1 000-grain weight and yield per plant.The results showed that RNAi-induced silencing of TaJIP2 significantly promoted the growth,early maturity and high yield of wheat,which indicated that TaJIP2 was a key negative regulator of wheat growth,maturity and yield.These findings provided genetic resources and research basis for promoting the photosynthetic efficiency,growth,early ripeness and high yield in wheat.

Key words: Wheat, Triticum aestivum jasmonate-induced protein 2(TaJIP2), RNA interference, Growth promotion, Early ripeness, High yield

CLC Number:

S512.01
Q78

WANG Xiaoya, BU Ruifang, HU Haiyan, LONG Qiang, SUN Lianxuan, QI Lu, LIU Zheng, LI Xiaoyao, LI Chengwei. Cloning and Function Analysis of TaJIP2 Gene Negatively Regulating Growth, Maturity and Yield in Triticum aestivum L.[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2021, 36(6): 35-44. doi: 10.7668/hbnxb.20192370.

/ / Recommend / Download Citations

URL: http://www.hbnxb.net/EN/10.7668/hbnxb.20192370

http://www.hbnxb.net/EN/Y2021/V36/I6/35

References

[1] 傅向东,刘倩,李振声,张爱民,凌宏清,童依平,刘志勇.小麦基因组研究现状与展望[J].中国科学院院刊,2018,33(9):909-914.doi:10.16418/j.issn.1000-3045.2018.09.003.
Fu X D,Liu Q,Li Z S,Zhang A M,Ling H Q,Tong Y P,Liu Z Y.Research achievement and prospect development on wheat genome[J]. Bulletin of the Chinese Academy of Sciences,2018,33(9):909-914.
[2] 中华人民共和国国家统计局.国际统计年鉴[M].北京:中国统计出版社,2019:239-244.
National Bureau of Statistics of China.International statistical yearbook[M].Beijing:China Statistics Press,2019:239-244.
[3] 中华人民共和国国家统计局.中国统计年鉴[M].北京:中国统计出版社,2020:377-387.
National Bureau of Statistics of China.China statistical yearbook[M].Beijing:China Statistics Press,2020:377-387.
[4] Turner J G,Ellis C,Devoto A.The jasmonate signal pathway[J]. The Plant Cell,2002,14(S1):153-164.doi:10.1105/tpc.000679.
[5] Staswick P E,Su W,Howell S H.Methyl jasmonate inhibition of root growth and induction of a leaf protein are decreased in an Arabidopsis thaliana mutant[J]. Proceedings of the National Academy of Sciences of the United States of America,1992,89(15):6837-6840.doi:10.1073/pnas.89.15.6837.
[6] Fang H X,Luo F,Li P X,Zhou Q,Zhou X,Wei B D,Cheng S C,Zhou H S,Ji S J.Potential of jasmonic acid(JA) in accelerating postharvest yellowing of broccoli by promoting its chlorophyll degradation[J]. Food Chemistry,2020,309:125737.doi:10.1016/j.foodchem.2019.125737.
[7] Zeng X C,Zhou X,Zhang W,Murofushi N,Kitahara T,Kamuro Y.Opening of rice floret in rapid response to methyl jasmonate[J]. Journal of Plant Growth Regulation,1999,18(4):153-158.doi:10.1007/PL00007063.
[8] Liu Y,Wang H Y.JA modulates phytochrome a signaling via repressing FHY3 activity by JAZ proteins[J]. Plant Signaling & Behavior,2020,15(3):1726636.doi:10.1080/15592324.2020.1726636.
[9] 王俊斌,李欣,王海风,任健,张少英.茉莉酸对植物花器官发育和叶片衰老的分子调控研究进展[J].天津农学院学报,2009,16(3):40-43.doi:10.3969/j.issn.1008-5394.2009.03.012.
Wang J B,Li X,Wang H F,Ren J,Zhang S Y.Molecular regulation progresses of jasmonic acid on plant flower organs development and leaves senescence[J]. Journal of Tianjin Agricultural College,2009,16(3):40-43.
[10] Pe n a-Cortés H,Barrios P,Dorta F,Polanco V,Sánchez C,Sánchez E,Ramírez I.Involvement of jasmonic acid and derivatives in plant response to pathogen and insects and in fruit ripening[J]. Journal of Plant Growth Regulation,2004,23(3):246-260.doi:10.1007/s00344-004-0035-1.
[11] Chehab E W,Kaspi R,Savchenko T,Rowe H,Negre-Zakharov F,Kliebenstein D,Dehesh K.Distinct roles of jasmonates and aldehydes in plant-defense responses[J]. PLoS One,2008,3(4):e1904.doi:10.1371/journal.pone.0001904.
[12] Li L,Zhao Y F,McCaig B C,Wingerd B A,Wang J H,Whalon M E,Pichersky E,Howe G A.The tomato homolog of CORONATINE-INSENSITIVE1 is required for the maternal control of seed maturation,jasmonate-signaled defense responses,and glandular trichome development[J]. The Plant Cell,2004,16(1):126-143.doi:10.1105/tpc.017954.
[13] Wasternack C,Stenzel I,Hause B,Hause G,Kutter C,Maucher H,Neumerkel J,Feussner I,Miersch O.The wound response in tomato-role of jasmonic acid[J]. Journal of Plant Physiology,2006,163(3):297-306.doi:10.1016/j.jplph.2005.10.014.
[14] 刘庆霞,李梦莎,国静.茉莉酸生物合成的调控及其信号通路[J].植物生理学报,2012,48(9):837-844.doi:10.13592/j.cnki.ppj.2012.09.013.
Liu Q X,Li M S,Guo J.Regulation of jasmonic acid biosynthesis and jasmonic acid signaling pathway[J]. Plant Physiology Communications,2012,48(9):837-844.
[15] 董桃杏,蔡昆争,曾任森.干旱胁迫下茉莉酸甲酯对水稻叶片质膜透性及无机离子含量的影响[J].华北农学报,2010,25(1):136-140.doi:10.7668/hbnxb.2010.01.027.
Dong T X,Cai K Z,Zeng R S.Effects of methyl jasmonate on membrane permeability and inorganic ions content in rice leaves under drought stress[J]. Acta Agriculturae Boreali-Sinica,2010,25(1):136-140.
[16] Sembdner G,Parthier B.The biochemistry and the physiological and molecular actions of jasmonates[J]. Annual Review of Plant Physiology and Plant Molecular Biology,1993,44(1):569-589.doi:10.1146/annurev.pp.44.060193.003033.
[17] Staswick P E,Huang J F,Rhee Y.Nitrogen and methyl jasmonate induction of soybean vegetative storage protein genes[J]. Plant Physiology,1991,96(1):130-136.doi:10.1104/pp.96.1.130.
[18] Hause B,Demus U,Teichmann C,Parthier B,Wasternack C.Developmental and tissue-specific expression of JIP-23,a jasmonate-inducible protein of barley[J]. Plant and Cell Physiology,1996,37(5):641-649.doi:10.1093/oxfordjournals.pcp.a028993.
[19] Andresen I,Becker W,Schlüter K,Burges J,Parthier B,Apel K.The identification of leaf thionin as one of the main jasmonate-induced proteins of barley(Hordeum vulgare)[J]. Plant Molecular Biology,1992,19(2):193-204.doi:10.1007/BF00027341.
[20] Xu Y,Chang P F L,Liu D,Narasimhan M L,Raghothama K G,Hasegawa P M,Bressan R A.Plant defense genes are synergistically induced by ethylene and methyl jasmonate[J]. The Plant Cell,1994,6(8):1077-1085.doi:10.1105/tpc.6.8.1077.
[21] Ryan C A.The search for the proteinase inhibitor-inducing factor,PIIF[J]. Plant Molecular Biology,1992,19(1):123-133.doi:10.1007/BF00015610.
[22] 王飞,田雪军,赵月,臧新山,耿晓丽,倪中福,姚颖垠,胡兆荣,辛明明.小麦茉莉酸诱导蛋白基因TaJIP的克隆和功能鉴定[J].中国农业大学学报,2015,20(2):50-57.doi:10.11841/j.issn.1007-4333.2015.02.07.
Wang F,Tian X J,Zhao Y,Zang X S,Geng X L,Ni Z F,Yao Y Y,Hu Z R,Xin M M.Isolation and characterization of jasmonate induced protein TaJIP in wheat(Triticum aestivum)[J]. Journal of China Agricultural University,2015,20(2):50-57.
[23] 牛吉山,常阳,王保勤.一个小麦茉莉酮酸酯诱导蛋白基因的克隆和鉴定[J].植物病理学报,2008,38(2):171-177.doi:10.3321/j.issn:0412-0914.2008.02.009.
Niu J S,Chang Y,Wang B Q.Cloning and characterization of a jasmonate-induced protein gene in wheat[J]. Acta Phytopathologica Sinica,2008,38(2):171-177.
[24] Supartana P,Shimizu T,Shioiri H,Nogawa M,Nozue M,Kojima M.Development of simple and efficient in planta transformation method for rice (Oryza sativa L.) using Agrobacterium tumefaciens[J]. Journal of Bioscience and Bioengineering,2005,100(4):391-397.doi:10.1263/jbb.100.391.
[25] Hummon A B,Lim S R,Difilippantonio M J,Ried T.Isolation and solubilization of proteins after TRIzol extraction of RNA and DNA from patient material following prolonged storage[J]. BioTechniques,2007,42(4):467-470,472.doi:10.2144/000112401.
[26] 向阳,杜才富,马正强.Jacalin类凝集素研究进展[J].植物生理学报,2013,49(10):1023-1029.doi:10.13592/j.cnki.ppj.2013.10.017.
Xiang Y,Du C F,Ma Z Q.Progress in research on jacalin-related lectins[J]. Plant Physiology Journal,2013,49(10):1023-1029.
[27] Xiang Y,Song M,Wei Z Y,Tong J H,Zhang L X,Xiao L T,Ma Z Q,Wang Y.A jacalin-related lectin-like gene in wheat is a component of the plant defence system[J]. Journal of Experimental Botany,2011,62(15):5471-5483.doi:10.1093/jxb/err226.
[28] Ma Q H,Zhen W B,Liu Y C.Jacalin domain in wheat jasmonate-regulated protein Ta-JA1 confers agglutinating activity and pathogen resistance[J]. Biochimie,2013,95(2):359-365.doi:10.1016/j.biochi.2012.10.014.
[29] Yong W D,Xu Y Y,Xu W Z,Wang X,Li N,Wu J S,Liang T B,Chong K,Xu Z H,Tan K H,Zhu Z Q.Vernalization-induced flowering in wheat is mediated by a lectin-like gene VER2[J]. Planta,2003,217(2):261-270.doi:10.1007/s00425-003-0994-7.
[30] 程燕.小麦凝激素类基因TaJRL2 的克隆与分析[D].南京:南京农业大学,2010:38-45.doi:10.7666/d.Y2527512.
Cheng Y.Cloning and analysis of wheat lectin-like gene TaJRL2[D].Nanjing:Nanjing Agricultural University,2010:38-45.
[31] Görlach J,Volrath S,Knauf-Beiter G,Hengy G,Beckhove U,Kogel K H,Oostendorp M,Staub T,Ward E,Kessmann H,Ryals J.Benzothiadiazole,a novel class of inducers of systemic acquired resistance,activates gene expression and disease resistance in wheat[J]. The Plant Cell,1996,8(4):629-643.doi:10.1105/tpc.8.4.629.
[32] Subramanyam S,Sardesai N,Puthoff D P,Meyer J M,Nemacheck J A,Gonzalo M,Williams C E.Expression of two wheat defense-response genes, Hfr-1 and Wci-1,under biotic and abiotic stresses[J]. Plant Science,2006,170(1):90-103.doi:10.1016/j.plantsci.2005.08.006.
[33] 陈同睿,罗艳君,赵潘婷,贾海燕,马正强.过表达TaJRL53 基因提高了小麦赤霉病抗性[J].作物学报,2021,47(1):19-29.doi:10.3724/SP.J.1006.2021.01050.
Chen T R,Luo Y J,Zhao P T,Jia H Y,Ma Z Q.Overexpression of TaJRL53 enhances the Fusarium head blight resistance in wheat[J]. Acta Agronomica Sinica,2021,47(1):19-29.

Please choose a citation manager

Content to export