Amino Acid Sequence Analysis of Mycoplasma hyopneumoniae P36, P46, P97 R1 and P146 in Slaughter Pigs

doi:10.7668/hbnxb.201751207

Abstract

Abstract: To understand the genetic variation of Mycoplasma hyopneumoniae (M.hyo) epidemic strain, a conventional PCR was employed to amplify the fragments of M. hyo P36, P46, P97 R1 and P146 gene from the DNA mix of bronchoalveolar lavage fluid of 17 slaugher pigs, which had been tested positive to M.hyo, and the PCR products were sequenced directly and analyzed. The deduced amino acid sequences of P36 and P46 were conservative between M.hyo of 17 slagughter pig lung samples and those strains registered in GenBank, with a homology of more than 98.9%. Among the amino acid sequences, there were large variations in P97 R1 area and 2 repetitive sequence regions (R1, R2) of P146. P97 amino acid sequences R1 area of M.hyo from 11 slaughter pig samples and 5 strains registered in GenBank were composed of 14 or more tandem copies. It was composed of 9-11 tandem copies in three vaccine-related strains(168 strain, 168-L strain, J strain), four other strains registered in GenBank, and 4 strains from slaughter pigs. A few amino acid deficiency was observed in R1 area of P146 from 5 strains registered in GenBank (168 strain, 168-L strain, J strain, and two other strains) and 1 slaughter pig samples, and 3-9 amino acid deficiency was observed in R2 area of P146 from 11 strains (168-L strain, 168 strain, three other strains registered in GenBank, and 6 strains detected from slaughter pig). Consequently, antigen epitope prediction showed significant difference in P97 R1 area and two repetitive sequence regions (R1, R2) of P146. To the best of our knowledge, this study was the first preliminary investigation to M.hyo strain in slaughter pigs in Hunan province, and it could provide some significant data for understanding the prevalence of M.hyo strain in different areas and screening of vaccine candidate strains.

Key words: Mycoplasma hyopneumoniae, P36, P46, P97, P146, PCR, Difference analysis

CLC Number:

S852.62

LI Runcheng, FANG Chao, QING Renke, GE Meng, ZHAO Dun, HU Yuli, YU Xinglong. Amino Acid Sequence Analysis of Mycoplasma hyopneumoniae P36, P46, P97 R1 and P146 in Slaughter Pigs[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2019, 34(2): 212-220. doi: 10.7668/hbnxb.201751207.

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