Studies on Diversity of Rhizosphere Microorganism in Quinoa Continuous Cropping Soil by High Throughput Sequencing

doi:10.7668/hbnxb.201751218

Abstract

Abstract: In order to reveal the changes of bacterial community structure and diversity in quinoa continuous cropping soil, the V4 aera of 16S rRNA gene of bacteria from the quinoa rhizosphere soil with different treatments (planting for 1 a and for 2 a) was sequenced by high throughput sequencing. The results showed that the abundance and diversity index of the bacterial community in rhizosphere soil of planting quinoa for 1 a were higher than those of planting quinoa for 2 a, and the average values of Chao1, ACE and Shannon indexes increased by 16.4%, 22.9% and 2.3%, respectively. The taxonomic composition analysis of bacterial community showed that, after continuous cropping of quinoa, the bacterial diversity of Lentzea, Lysobacter, Mesorhizobium and Polaromonas increased, but the bacterial diversity of Mycobacterium, Luteimonas, Gemmatimonas and Planctomyces decreased. The analysis of functional prediction showed that the functional genes that code the membrane transport, the translation, copy and repair, the degradation and metabolism of exogenous substances and the biosynthesis and metabolism of polysaccharide decreased sigenificantly, and the functional genes coding the metabolism of nucleotide, steroids, cofactors and vitamin also decreased a little, but the functional genes of coding signal transduction and lipid metabolism increased in a small amount.

Key words: Quinoa, Continuous cropping, High-throughput sequencing, Rhizosphere soil, Microbial diversity

CLC Number:

S144

DONG Yanhui, YU Yufeng, WEN Xin, WANG Yixue, NIE Yuanjun, HOU Liyuan, LI Yali, LIU Jiang, REN Yuan, WANG Yuchuan, CAO Qiufen, WU Shenjie, WANG Bin, QIN Yongjun. Studies on Diversity of Rhizosphere Microorganism in Quinoa Continuous Cropping Soil by High Throughput Sequencing[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2019, 34(2): 205-211. doi: 10.7668/hbnxb.201751218.

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