Effect Substitution of Chemical Fertilizer with Microbial Fermentation Solution Cai-18 on Growth of Cucumber and Rhizospheric Ecology in Greenhouse Fertile Soil

doi:10.7668/hbnxb.20190824

Abstract

Abstract: To improve soil ecological environment of greenhouse vegetables and promote the implementation of Zero Increment Plan for Chemical Fertilizer. Three fertilization treatments including chemical fertilizer(WSF),Cai-18 microbial fermentation liquid(Cai-18) and no-fertilizer (CK) were chosen in green house fertile soil with the content of available N 283.55 mg/kg,P 574.05 mg/kg,K 1 161.3 mg/kg and organic matter 54.9 g/kg. Rhizosphere soil micro-flora,soil nutrient content,quality and yield of cucumber grown on fertile soil with the above three fertilization treatments in greenhouse were tested by using Miseq high-throughput technique,GC-MS determination,HS-SPME-GCMS analysis,as well as other general testing methods. The results showed that there was no significant difference in cucumber yield between WSF and Cai-18 treatments whose consumption in N,P,K was reduced by 93.48%,95.44% and 98.63% compared with the former,while the cucumber yield was 6.21% and 6.59% higher than that of CK(P <0.05),respectively. These two treatments had no difference in the fruit texture and content of soluble sugar,while the contents of soluble sugar were significantly higher than that of CK. Comparably,more species and higher relative contents of esters could be produced by using Cai-18 microbe fermentation liquid with a result of facilitating fruit favor. The indexes of OTU,Chao 1 and Shannon in bacterial flora of rhizosphere soil treated by Cai-18 microbial fermentation liquid were higher than those treated by WSF and CK,but Chao 1 and Shannon indexes were lower in fungi flora.Cai-18 treatment improved quantity homogeneity of bacterial and fungi flora in rhizosphere soil,while CK treatment ameliorated fungi flora homogeneity. The content of available K in Cai-18 treatment,was 140.85 mg/kg lower than that of WSF treatment in soil after harvest (P <0.05),while there was no significant difference in the content of available P between these two treatments. As compared with CK,the treatments of Cai-18 and WSF gained significantly higher content of available K and P. It could be concluded that as compared with chemical fertilizer,Cai-18 microbe fermentation liquid on fertile soil could improve quality of product and balance structure of micro-flora and nutrition in soil with no-reducing cucumber yield.

Key words: Cucumber, Cai-18 microbe fermentation liquid, Rhizosphere soil micro-flora, Nutrient content in root soil, Yield, Quality

CLC Number:

S143

YANG Xiaoling, TONG Yana, HUA Mingyan, SONG Lanfang, MA Hongying, ZHANG Shuxiang. Effect Substitution of Chemical Fertilizer with Microbial Fermentation Solution Cai-18 on Growth of Cucumber and Rhizospheric Ecology in Greenhouse Fertile Soil[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2020, 35(4): 153-160. doi: 10.7668/hbnxb.20190824.

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