Karyotype Analysis of C.quinoa Varieties with Different Seed Color

doi:10.7668/hbnxb.20191704

Abstract

Abstract: In order to determine the chromosome number and karyotype characteristics of the main Chenopodium varieties in Qinghai Province,three quinoa varieties with different seed colors Qaidam black-1(abbreviated as black quinoa),Qaidam white-1(abbreviated as white quinoa)and Qaidam red-1(abbreviated as red quinoa)were used as materials,and their root tips were sliced by using squashing method.Karyotype analysis was carried out through the steps of ice-water mixture pretreatment,Carnot fixed solution,45% acetic acid hydrolysis,flame drying and pressing,microscopic examination,DAPI staining,fluorescence microscope photography etc.The results showed that:The chromosome numbers of the three color varieties were all 4n=36,and no aneuploid or polyploid phenomenon was found.The karyotype formula and the relative length composition of chromosome of black quinoa were 4n=36=32m(4AST)+ 4sm,4n=36=2L+18M2+12M1+ 4S;the karyotype formula and the relative length of chromosome of white quinoa were 4n=36=34m(4SAT)+ 2sm,4n=36=2L+20M2+14M1;the karyotype formula and the relative length of chromosome of red quinoa were 4n=36=34m(4SAT)+2sm,4n=36=6L+8M2+14M1+8S.All varieties belong to 2B type and the asymmetry index were 58.20%,58.22% and 58.72%,respectively,indicating that these varieties were more evolved.All the three varieties have two pairs of satellite,but there were differences in loction,which may be related to the heterologous origin of quinoa,self-pollination and other factors.This study can provide cytological basis for cross breeding,ploidy breeding,germplasm resource identification and gene mapping of C.quinoa.

Key words: Chenopodium quinoa Willd., Chromosome, Karyotypic analysis, Seed color

CLC Number:

S512.03

QUAN Youjuan, LIU Bo, YUAN Feimin, LI Xiang, CHEN Zhiguo. Karyotype Analysis of C.quinoa Varieties with Different Seed Color[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2020, 35(S1): 72-77. doi: 10.7668/hbnxb.20191704.

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