Full-length Transcriptome Analysis of Fruit Cracking of Chinese Jujube under Water Stress

doi:10.7668/hbnxb.20191835

Abstract

Abstract: In order to illuminate the mechanisms of fruit cracking of Chinese jujube under water stress,5 a Jinsi novel No.4 Chinese jujube was chosen.The trees were spayed for 50 hours to simulate water stress.Fruits before and after treatment were sequenced on the ONT third-generation sequencing platform.There were totally 13 368 transcripts obtained from 6 samples.9 178 PolyA sites were identified in the APA event.78 significantly enriched PolyA signals(PAS)motif were found.7 321 SSRs and 114 lncRNAs were predicted.239,189 alternative splicing events were identified in the control and treatment group,respectively.There were 248 differentially expressed transcripts(DETs)between the two groups.DETs mainly encoded calnexin,calreticulin,heatshock proteins, microtube proteins fructokinase and so on.GO enrichment showed that DETs were mainly involved in the biological processes such as translation,positive regulation of mitotic centrosome separation and cold acclimation.Cellular components participating those processes included cytosol and cytosolic large ribosomal subunit etc.Enriched molecular functions included structural constituent of ribosome and xyloglucosyl transferase activity,etc.KEGG enrichment analysis showed that up-regulated DETs were involved in protein processing in endoplasmic reticulum,phagosome,amino sugar and nucleotide sugar metoboism,starch and sucrose metabolism,etc.Down-regulated DETs took part in protein processing in endoplasmic reticulum,nitrogen metabolism,photosynthesis-antenna proteins and so on.The mechanisms of fruit cracking of Chinese jujube under water stress might include decrease of alternative splicing events,regulation of Ca²⁺ by calnexin and calreticulin,up-regulation of heat shock proteins resulting from cold stress by continuous spaying,and up-regulation of cell wall biosynthesis related transcripts.

Key words: Chinese jujube, Fruit cracking, Water stress, Full-length transcriptome, Calnexin, Heatshock protein

CLC Number:

Q78
S665.03

WEI Wei, JIA Yanli, WU Shuo, ZHI Fujun. Full-length Transcriptome Analysis of Fruit Cracking of Chinese Jujube under Water Stress[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2020, 35(S1): 63-71. doi: 10.7668/hbnxb.20191835.

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