Construction of Single Segment Substitution Lines Confer Resistance to Maize Rough Dwarf Disease Based on Meta-analysis

doi:10.7668/hbnxb.2016.06.009

Abstract

Abstract: On the basis of the high-density linkage map of maize IBM2 2008 Neighbors,92 QTLs (Quantitative trait locus) associated with maize rough dwarf disease were collected and an integrated QTL map were constructed.By using Meta-analysis,24 Meta-QTLs were predicted and their physical locations were estimated through bioinformatics analysis.Based on the whole genome resequencing of two maize inbred line Qi 319 and Zheng 58,7 142 InDel loci were identified at the Meta-QTL region and 546 pairs primers were designed for PCR analysis.Finally 158 InDel markers were screened for their polymorphism between Qi 319 and Zheng 58,and distributed on chromosome 2,4,5,6,7,8,and 10,respectively.With these InDel marker assisted selection,a series of SSSLs(Single segment substitution lines) were constructed by introduce these QTLs from resistant maize line Qi 319 (Donor parent) into the susceptible line Zheng 58(Recipient parent).These SSSLs will be benefit for fine-mapping of QTLs associated with maize rough dwarf disease and also provide new resources for resistance breeding.

Key words: Maize, Maize rough dwarf disease, Meta-analysis, Chromosome single segment substitution lines

CLC Number:

Q78
S435

BAO Huabin, LIANG Shuaiqiang, LIN Feng. Construction of Single Segment Substitution Lines Confer Resistance to Maize Rough Dwarf Disease Based on Meta-analysis[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2016, 31(6): 56-62. doi: 10.7668/hbnxb.2016.06.009.

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