Mapping of QTLs foR GRain Weight Using ChRomosome Single Segment Substitution Lines in Rice

doi:10.7668/hbnxb.2013.06.003

Abstract

Abstract: Grain weight is one of major factors determining rice yield, which is a typical quantitative trait con-trolled by multiple genes.With Guangluai 4 as recipient and Nipponbare as donor, a population of 119 chromosome segment substitution lines had been developed.Correlation analysis between grain weight and grain shape by SPSS revealed that 1000-grain weight was significantly and positively correlated with grain length and length -width ratio, while showed no correlation with grain width and thickness.The QTL analysis of grain weight was carried out using one-way analysis of variance and Dunnett's test.Nineteen stable QTLs for grain weight were identified over two years.All 19 QTLs were identified on all chromosomes except for chromosome 10 and 12 with a significant level of P≤0.001.Among them,10 QTLs had a positive effect and derived from the Nipponbare allele, the additive effect of these QTLs ranged from 0.49 g to 2.74 g,the additive effect percentages ranged from 2.00%to 11.05%.Addition-al 9 QTLs had a negative effect and were all derived from Guangluai 4 alleles,the additive effect of these QTLs ranged from 0.60 g to 2.35 g,the additive effect percentages ranged from 2.40%to 9.84%.The results provide a basis for the fine mapping and gene cloning of novel locus associated with rice grain weight.

Key words: Rice, ChRomosome single segment substitution lines, Quantitative tRait loci, 1000-gRain weight

CLC Number:

S511

WANG Jun, ZHOU Yong, YANG Jie, ZHU Jin-yan, FAN Fang-jun, LI Wen-qi, LIANG Guo-hua, ZHONG Wei-gong. Mapping of QTLs foR GRain Weight Using ChRomosome Single Segment Substitution Lines in Rice[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2013, 28(6): 11-17. doi: 10.7668/hbnxb.2013.06.003.

/ / Recommend / Download Citations

URL: http://www.hbnxb.net/EN/10.7668/hbnxb.2013.06.003

http://www.hbnxb.net/EN/Y2013/V28/I6/11

References

[1] 马丽莲,郭龙彪,钱前.水稻大粒种质资源和遗传分析[J].植物学通报, 2006,23( 4): 395-401.
[2] 姚国新,卢磊.水稻粒重基因定位克隆研究[J].安徽农业科学, 2007,35( 27): 8468,8478.
[3] Ishimaru K,Hirotsu N,Madoka Y, et al.Loss of function of the IAA-glucose hydrolase gene TGW6 enhances rice grain weight and increases yield[J].Nat Genet,2013, doi: 10.1038/ng.2612.
[4] Fan C C,Xing Y Z,Mao H L, et al.GS3,a major QTL for grain length and weight and minor QTL for grain width and thickness in rice,encodes a putative transmembrane protein[J].Theor Appl Genet,2006,112 ( 6 ): 1164 －1171.
[5] Song X J,Huang W,Shi M, et al.A QTL for rice grain width and weight encodes a previously unknown RING type E3 ubiquitin ligase[J].Nat Genet,2007,39 ( 5):623-630.
[6] Shomura A, Izawa T,Ebana K, et al.Deletion in a gene associated with grain size increased yields during rice domestication[J].Nat Genet,2008,40( 8): 1023-1028.
[7] Weng J F,Gu S H,Wan X Y, et al.Isolation and initial characterization of GW5,a major QTL associated with rice grain width and weight[J].Cell Res,2008,18( 12): 1199 －1209.
[8] Li Y B,Fan C C,Xing Y Z, et al.Natural variation in GS5 plays an important role in regulating grain size and yield in rice[J].Nat Genet,2011,43( 12): 1266-1269.
[9] Wang S K,Wu K,Yuan Q B, et al.Control of grain size,shape and quality by OsSPL16 in rice[J].Nat Genet, DOI: 10.1038/ng.2327.
[10] Hu Z J,He H H,Zhang S Y, et al.A kelch motif-containing serine/threonine protein phosphatase determines the large grain QTL trait in rice[J].Journal of Integrative Plant Biology,2012,54( 12): 979-990.
[11] Qi P,Lin Y S,Song X J,et al.The novel quantitativetrait locus GL3.1 controls rice grain size and yield by regulating Cyclin-T1; 3 [J].Cell Research,2012,22( 12): 1666-1680.
[12] Zhang X J,Wang J F,Huang J, et al.Ｒare allele of OsPPKL1 associated with grain length causes extra-large grain and a significant yield increase in rice[J].Proc Natl Acad Sci USA,2012,109( 52): 21534-21539.
[13] Li J M,Thomson M,McCouch S R.Fine Mapping of a grain-weight quantitative trait locus in the pericentromeric region of rice chromosome 3[J].Genetics,2004,168:2187-2195.
[14] Xie X B,Song M H, Jin F X, et al.Fine mapping of agrain weight quantitative trait locus on rice chromosome 8 using near-isogenic lines derived from a cross betweenOryza sativa and Oryza rufipogon[J].Theor Appl Genet, 2006,113: 885-894.
[15] Oh J M,Balkunde S,Yang P,et al.Fine mapping of grain weight QTL,tgw11 using near isogenic lines from a cross between Oryza sativa and O.grandiglumis[J].Genes ＆ Genomics,2011,33: 259-265.
[16] Oh J M,Yoon D B,Ahn S N.Fine mapping of grain weight QTLs using near isogenic lines from a cross between Oryza sativa and O.grandiglumis.J[J].Crop Sci Biotech,2010,13( 1): 7-12.
[17] Guo L B,Ma L L, Jiang H, et al.Genetic analysis and fine mapping of two genes for grain shape and weight in rice[J].Journal of Integrative Plant Biology,2009,51( 1): 45-51.
[18] Zhang Q,Yao G X,Hu G L, et al.Fine mapping of qTGW3-1,a QTL for 1000-grain weight on chromosome 3 in rice[J].Journal of Integrative Agriculture,2012,11( 6): 879-887.
[19] Shao G N,Wei X J,Chen M L, et al.Allelic variation for a candidate gene for GS7,responsible for grain shape in rice[J].Theor Appl Genet,2012,125 ( 6 ): 1303 －1312.
[20] 吴秀菊,万向元,江玲,等.多环境下稻米粒重的QTL 定位[J].作物学报, 2007,33( 11): 1771-1776.
[21] 赵芳明,朱海涛,丁效华,等.基于SSSL 的水稻重要性状QTL 的鉴定及稳定性分析[J].中国农业科学,2007,40( 3): 447-456.
[22] 姚国新,李金杰,张强,等.利用4 个姊妹近等基因系群体定位水稻粒重和粒形QTL[J].作物学报,2010,36( 8): 1310-1317.
[23] Sun L,Ma D P,Yu H H, et al.Identification of quantitative trait loci for grain size and the contributions of major grain-size QTLs to grain weight in rice[J].Molecular Breeding,2013,31( 2): 451-461.
[24] 陈冰嬬,石英尧,崔金腾,等.利用BC2F2高代回交群体定位水稻籽粒大小和形状QTL[J].作物学报,2008,34( 8): 1299-1307.
[25] 吴家胜,周鸿凯,陈国波,等.水稻单穗重和千粒重的QTL 定位[J].中国水稻科学,2008,22 ( 2 ): 143 －147.
[26] 刘冠明,李文涛,曾瑞珍,等.水稻单片段代换系代换片段的QTL 鉴定[J].遗传学报, 2004,31( 12): 1395 －1400.
[27] Eshed Y,Zamir D.An introgression line population of Lycopersicon pennellii in the cultivated tomato enables the identification and fine mapping of yield-associated QTL[J].Genetics,1995,141: 1147-1162.
[28] McCouch S R,CGSNL( Committee on Gene Symbolization,Nomenclature and Linkage,Rice Genetics Cooperative).Gene nomenclature system for rice[J].Rice,2008,1( 1): 72-84.
[29] Paterson A H,Deverna J W,Lanini B, et al.Fine mapping of quantitative trait loci using selected overlapping recombinant chromosomes in an interspecies cross of tomato[J].Genetics,1990,124: 735-742.
[30] Young N D,Tanksley S D.Restriction fragment length polymorphism maps and the concept of graphical genotypes[J].Theor Appl Genet,1989,77( 1): 95-101.
[31] 张亚东,赵庆勇,陈涛,等.一种特大粒型水稻粒重遗传的初步研究[J].江苏农业学报,2008,24 ( 增刊): 32-36.
[32] 芮重庆,赵安常.籼稻粒重及粒形性状F1遗传特性双列分析[J].中国农业科学, 1983( 5): 14-20.
[33] 石春海,申宗坦.早籼粒形的遗传和改良[J].中国水稻科学, 1995,9( 1): 27-32.
[34] 王余龙,姚友礼,李昙云,等.水稻籽粒性状与粒重关系的初步探讨[J].作物学报,1995,21 ( 5): 573 －578.
[35] 符福鸿,王丰,黄文剑,等.杂交水稻谷粒性状的遗传分析[J].作物学报, 1994,20( 1): 39-45.
[36] 张颖慧,谢永楚,董少玲,等.利用水稻籼粳重组自交系群体研究粒型性状与千粒重的相关性[J].江苏农业学报, 2012,28( 2): 231-235.
[37] Mao D H,Liu T M,Xu C G, et al.Epistasis and complementary gene action adequately account for the genetic bases of transgressive segregation of kilo-grain weight in rice[J].Euphytica,2011,180( 2): 261-271.
[38] Liu T M,Shao D,Kovi M R, et al.Mapping and validation of quantitative trait loci for spikelets per panicle and 1, 000-grain weight in rice( Oryza sativa L.) [J].Theor Appl Genet,2010,120( 5): 933-942.
[39] Liu T M,Zhang Y S,Xue W Y,et al.Comparison of quantitative trait loci for 1, 000-grain weight and spikelets per panicle across three connected rice populations[J].Euphytica,2010,175( 3): 383-394.
[40] Marathi B,Guleria S,Mohapatra T, et al.QTL analysis of novel genomic regions associated with yield and yield related traits in new plant type based recombinant inbred lines of rice( Oryza sativa L.) [J].BMC Plant Biology,2012,12: 137-155.

Please choose a citation manager

Content to export