MoleculaR Cloning,ExpRession and BioinfoRmatic Analysis of TaCBL7 fRom Wheat

doi:10.7668/hbnxb.2013.06.004

Abstract

Abstract: The cDNA sequence of TaCBL7 were isolated and analyzed from wheat EST database.The results showed that TaCBL7 contained a 642 bp ORF encoding 213 amino acids.Using the methods of bioinformatics to predict and analyze TaCBL7, the protein has the high level homology with OsCBL 7 and highly conserved domain of EF-hand and N-myristoylation.The results of Real-time PCR reveal that the expression of TaCBL7 has expression in leaf,stem,seed and root both seedling and mature stage.In addition,the expression of TaCBL7 was induced by drought,salt,low-temperature,ABA and yellow rust.Those results indicate that TaCBL7 has specific expression of temporal and spatial, and the expression was induced by biotic and abiotic stresses.It is speculated that TaCBL7 could play a role not only in growth and development conditions but also in stress of wheat.This investigation laid a foundation for functional dissection of TaCBL7 and wheat molecular breeding with TaCBL7 in the future.

Key words: Wheat, TaCBL7, Gene clone, Bioinformatic analysis/Gene expression

CLC Number:

S512.1

LONG Xiang-yu, PU Zhi-en, ZHENG Ke, LIU Ze-hou. MoleculaR Cloning,ExpRession and BioinfoRmatic Analysis of TaCBL7 fRom Wheat[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2013, 28(6): 18-23. doi: 10.7668/hbnxb.2013.06.004.

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