Abstract: A homologous gene of GH3 was obtained in Capsicum annuum by in silicon cloning, and some char-acters of the gene and its encoded protein were analyzed using the methods of bioinformatics in the following as -pects,including the general physical and chemical properties, hydrophobicity, three dimensional structure, subcellu-lar localization and phylogenetic relationships.The results showed the cDNA length of the gene was 2 140 bp long and contained a complete ORF ( 1 791 bp ) which encoded 595 amino acids.The deduced protein comprised 32 phosphorylation sites, no signal peptide and transmembrane helix.The GH3 of Capsicum annuum was a hydrophilic and chloroplast protein.The amino acid sequence of the gene showed high similarity with the homologous in other plants,such as Arabidopsis thaliana,Glycine max,Solanum lycopersicum,Nicotiana tabacum.The results laid a solid foundation for gene function analysis of GH3 in Capsicum annuum.

Key words: Capsicum annuum, GH3, In silico cloning, BioinfoRmatics

CLC Number: 

• S641.3