Cloning and ExpRession PatteRn Analysis of a PRoton TRanspoRteR GmMRP5 in Soybean

doi:10.7668/hbnxb.2013.06.006

Abstract

Abstract: In order to investigate the mechanism of the MRP protein genes in soybean, a novel soybean gene GmMRP5 was identified in soybean cultivar.GmMRP5 containing an open reading frame ( ORF) of 4 479 bp nucleo-tides.The ORF encoded a protein of 1 492 amino acids.Sequence alignment and evolutionary tree clustering analy-sis showed that GmMRP5 contains 13 putative transmembrane-spanning domains(TMDs)and ABC transport protein conserved domain.Phylogenetic tree analysis showed that Gm MRP5 was closely related to AtMRP5 and both be-longed to ABCC subfamily.GmMRP5 was a constitutive expression gene and expressed in root, stem and leaves by using Real-Time PCR analysis in soybean.GmMRP5 was strongly induced and highly expressed in root after treat-ment by proline and citrate solution.Real-Time PCR showed the expression level of GmMRP5 was increaced by dif-ferent pH buffer solution treatment, suggesting that GmMRP5 gene may be as a proton transporter to participate in the stress response in soybean.

Key words: Soybean, PRoton tRanspoRteR, Gene cloning, ExpRession analysis

CLC Number:

S565.1

XU Ling, ZHANG Da-yong, HE Xiao-lan, XU Zhao-long, LIU Xiao-qing, HUANG Yi-hong, MA Hong-xiang, YI Jin-xin. Cloning and ExpRession PatteRn Analysis of a PRoton TRanspoRteR GmMRP5 in Soybean[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2013, 28(6): 30-35. doi: 10.7668/hbnxb.2013.06.006.

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