Abstract: Mannose-binding Lectin(MBL),which belongs to B_LECTIN gene families,was isolated fom the young leaf of apple ( Malus domestica) by homologous cloning technology, named as MdMBL.The cDNA was 1 181 bp in length with an 5'non-coding egion of 20 bp,an 3'non-coding egion of 81 bp and an open eading fame ( OF) of 1 080 bp encoding a potein of 359 amino acids, and the estimated molecula weight and isoelectic point (pI)of the putative potein wee 39.76 kDa and 8.72,espectively.The phylogenetic tee of MBL showed that Md-MBL was closest with gapes MBL, followed by glycine max, and the evolutionay elationships of homologous genes in apple MdMBL2 was fathest.Quantitative eal-time PC analysis shows that the gene is tissue-specific and ex-pessed highest in stems, the lowest in flowes;The expession of MdMBL was pomoted inceased with the cold stess and the mechanical damage stess;Exogenous SA could induce up-egulation of MdMBL expession.

Key words: Malus domestica, Mannose-binding lectin( MBL), Gene cloning, Gene expession

CLC Number: 

• S661.1

• Q943