Alleviating Effect of External Application of 2,4-Epbrassinolide on Freezing Stress in Winter Wheat at Jointing Stage

doi:10.7668/hbnxb.20192445

Abstract

Abstract:

In order to investigate the effects of exogenous brassinolide on physiological characteristics,transcriptional level and growth of wheat at jointing stage under freezing stress,the semi-winter wheat variety Bainong 207 was used as material,and irrigated with 0.1 mg/L 2,4-Epibrassinolide(EBR)to study its chlorophyll content(SPAD value),malondialdehyde(MDA)content,relative conductivity,free proline(Pro)content,stress resistance related gene expression,young panicle freezing rate,grain yield,1 000 grains weight and dry matter quality.The results showed that before freezing stress,there was no significant difference in SPAD value,MDA content and relative conductivity between the control group and the treatment group,but the Pro content in the treatment group was significantly lower than that in the control group;after freezing stress,compared with the control,external application of EBR significantly reduced the MDA content and relative conductivity of wheat leaves,and increased the SPAD value and Pro content.At 48 hours of freezing stress,the relative expression of stress resistance related genes SOD,POD,CAT,P5CS,and WCS120 in the treatment group were significantly higher than those in the control.After freezing stress,compared with the control,the freezing rate of young panicles in the treatment group decreased significantly by 20.93 percentage point,while the grains yield,1 000 grain weight and dry matter mass increased significantly by 19.38,2.37,54.40 g,respectively.In conclusion,external application of EBR at jointing stage of wheat can improve the frost resistance of wheat and reduce the yield loss of winter wheat caused by low temperature and freezing injury by reducing the content of MDA,increasing the content of Pro,chlorophyll and the expression of stress resistance related genes SOD,POD,CAT,P5CS,and WCS120.

Key words: Winter wheat, 2,4-Epbrassinolide, Freezing stress, Jointing stage

ZHANG Aoshen, YONG Xiaoyu, HAN Qiaoxia, YAO Yongwei, SHI Huanting, LI Gezi, KANG Guozhang. Alleviating Effect of External Application of 2,4-Epbrassinolide on Freezing Stress in Winter Wheat at Jointing Stage[J]. Acta Agriculturae Boreali-Sinica, 2022, 37(2): 78-83. doi: 10.7668/hbnxb.20192445.

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Figures/Tables 7

Tab.1 qRT-PCR primer information

基因名称 Gene name	基因登录号 GenBank ID	正向引物(5'—3') Forward primer(5'—3')	反向引物(5'—3') Reverse primer(5'—3')	扩增长度/bp Amplification length
SOD	FJ890986.1	TGACGCTGATGATCTTGGCAAGG	TAGCCCTGGAGCCCGATGATTC	99
POD	AF387866.1	GCCACAGCCACAGCCAGATAAC	CCACGAGCACCACCAGAGAAATG	118
CAT	D86327.1	TGTCAACACCTACACCTTAATCA	TTCATCATCCAAGAGGCACTTCA	100
P5CS	AF022914.1	GCACAAGACACATTGAAAGTAC	CAATCGCTGACTGCACATCATCAA	103
WCS120	M93342.2	GGCGTCATGGAGAACATCAAGGAG	GTGTCCGTGCTGCTCATAGGTG	147
Actin	AB181991	CGAAGCGACATACAATTCCATCATG	AGAACCTCCACTGAGAACAACATT	87

Fig.1 SPAD value in wheat leaves under freezing stress after external application of EBR at jointing stage
The different lowercase letters on the columns indicate significant differences at the 5% level.The same as Fig.2—5.

Fig.2 MDA content in wheat leaves under freezing stress after external application of EBR at jointing stage

Fig.3 Relative conductivities in wheat leaves under freezing stress after external application of EBR at jointing stage

Fig.4 Proline contents in wheat leaves under freezing stress after external application of EBR at jointing stage

Fig.5 Relative expression leaves of stress resistance related genes in wheat leaves under freezing stress after external application of EBR at jointing stage

Tab.2 The damage rates of young ears,grain yields,1 000 grains weights and dry matter qualities in wheat under freezing stress after external application of EBR at jointing stage

处理 Treatment	幼穗受冻率/% Young spike freezing rate	籽粒产量/(g/盆) Grain yield	千粒质量/g 1 000 grains weight	干物质质量/(g/盆) Dry mater weight
CK	69.84±3.22a	32.59±1.03b	40.72±0.75b	96.11±2.96b
EBR	48.91±1.54b	51.97±0.58a	43.09±0.87a	150.51±1.67a

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