Optimization of Chromosome Mounting Technique and Karyotype Analysis of Culinary Rhubarb

doi:10.7668/hbnxb.2013.05.023

Abstract

Abstract: In order to better understand the information about cytology of culinary rhubarb,provide information support for genetic breeding of culinary rhubarb. The protocol of different sampling time,pretreatments methods,dis-sociating time on chromosome observation from culinary rhubarb root tips was studied and karyotype of two cultivars ( RAMB and RO) was analyzed. The observation showed that root tips collecting at 8:00 am,pretreated in ice water mixture for 3 to 5min,dissocated in 1 mol/L HCl at 60℃for 15 min,dyed with modified carbolic acid-magenta for 3 to 5 min could achieve the optimal experimental results. Karyotype analysis of two cultivars of culinary rhubarb showed that the chromosome number was 44,with karyotype formula being 2n=4x=44=36m+8sm and 2n=4x=44=32m+12sm,respectively.The results demonstrated that the asymmetry indexes were 57.46% and 58.60%, respectively.The karyotype from the two cultivars of culinary rhubarb was 1B type and was symmetrical,suggesting that culinary rhubarb was relatively primitive forms.

Key words: Culinary rhubarb, Chromosome preparation, Karyotype analysis

CLC Number:

S644.9

REN Wen-juan, GUO Xiao-fei, JIANG Li-na, CAI Zu-guo, ZHAO Yi-peng. Optimization of Chromosome Mounting Technique and Karyotype Analysis of Culinary Rhubarb[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2013, 28(5): 128-132. doi: 10.7668/hbnxb.2013.05.023.

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