Construction of a Bacterial Artificial Chromosome Library from Brassica oleracea

doi:10.7668/hbnxb.2014.05.005

Abstract

Abstract: Bacterial artificial chromosome library is the foundation of important and high quality disease-resistant gene cloning from Brassica oleracea.A bacterial artificial chromosome library was constructed from R4P1,a Brassica oleracea varieties highly resisting to fusarium wilt and being in rich of glucosinolates,with the vector CopyControl^TM pCC1BAC^TM.The library consists of 73 344 clones with an average insert size of about 97 kb,and in which the empty clones are under 3%.Thus,the library provides 10.9 haploid genome equivalents,the probability of screening to any gene of B.oleracea is 99.99%,these results indicate that this is a high quality BAC library suitable for the following analysis.The BAC library of B.oleracea could not only be used for the clone of resistant genes to fusarium wilt,but also lay foundations for isolating other interesting genes and research the genomics of B.oleracea,et al.

Key words: Brassica oleracea, Bacterial artificial chromosome library, Fusarium wilt resistant gene, Clone

CLC Number:

XING Miao-miao, TIAN Duo-cheng, LI Hai-long, YAO Li-jun, JI Rui-qin, KANG Jun-gen. Construction of a Bacterial Artificial Chromosome Library from Brassica oleracea[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2014, 29(5): 23-28. doi: 10.7668/hbnxb.2014.05.005.

/ / Recommend / Download Citations

URL: http://www.hbnxb.net/EN/10.7668/hbnxb.2014.05.005

http://www.hbnxb.net/EN/Y2014/V29/I5/23

References

[1] Shizuya H,Birren B,Kim U J,et al.Clong and stable maintenance of 300-kilobase-pair fragments of human DNA in Escherichia coli using an F-factor-based vector [J].Proceedings of the National Academy Sciences of the USA,1992,89(18):8794-8797.
[2] 李海权,刁现民.基因组细菌人工染色体文库(BAC)的构建及应用[J].生物技术通报,2005(1):6-11.
[3] 王晓虹,金黎明.细菌人工染色体文库的构建及应用[J].生物技术通讯,2005,16(6):668-671.
[4] Probst F J,Fridell R A,Raphael Y,et al.Correction of deafness in shaker-2 mice by an unconventional myosin in a BAC transgene [J].Science,1998,280(5368):1444.
[5] Schibler L,Roiq A,Mahé M F,et al.A first generation bovine BAC-based physical map [J].Genetics Selection Evolution,2004,36(1):105-122.
[6] Jiang J M,Gill B S,Wang G L,et al.Metaphase and interphase fluorescence in situ hybridization mapping of the rice genome with bacterial artificial chromosomes [J].Proceedings of the National Academy Sciences of the USA,1995,92(10):4487-4491.
[7] 陶勇生,张祖新,程友林,等.水稻BAC在玉米有丝分裂染色体上FISH 杂交体系的构建[J].中国生物化学与分子生物学报,2007,23(1):80-84.
[8] Mozo T,Dewar K,Dunn P,et al.A complete BAC-based physical map of the Arabidopsis thaliana genome [J].Nature Genetics,1999,22(3):271-275.
[9] Mun J H,Kwon S J,Yang T J,et al.The first generation of a BAC-based physical map of Brassica rapa [J].BMC Genomics,2008,9:280.
[10] Zhu H,Blackmon B P,Sasimowski M,et al.Physical map and organization of chromosome 7 in the rice blast fungue, Magnaporthe grisea [J].Genome Research,1999,9(8):739-750.
[11] 林海艳.水稻基因组BAC物理图谱构建以及相关数据库、分析查询系统的本地搭建[D].武汉:华中农业大学,2011.
[12] Paux E,Sourdille P,Salse J,et al.A physical map of the 1-gigabase bread wheat chromosom3B[J].Science,2008,322(5898):101-104.
[13] Gonzalez J,Nefedov M,Bosdet I,et al.A BAC-based physical map of the Drosophila buzzatii genome[J].Genome Research,2005,15(6):885-892.
[14] Tanksley S D,GanalM W,Marti G B.Chromosome landing:a paradigm for map-based gene cloning in plants with large genomes[J].Trends in Genetics,1995,11(2):63-68.
[15] Song W Y,Wang G L,Chen L L,et al.A receptor kinase-like protein encoded by the rice dissease resistance gene, Xa21 [J].Science,1995,270(15):8405-8407.
[16] T Joobeur,G Gusmini,X Zhang,et al.Construction of a watermelon BAC library and identification of SSRs anchored to melon or Arabidopsis genomes[J].Theor Appl Genet,2006,112:1553-1562.
[17] Nam Y W,Lee J R,Song K H,et al.Construction of two BAC libraries from cucumber(Cucumis sativus L.) and identification of clones linked to yield component quantitative trait loci[J].Theoretical and Applied Genetics,2005,111(1):150-161.
[18] Yu Y,Tomkins J P,Waugh R,et al.A bacterial artificial chromosome library for barley(Hordeum vulgare L.) and the identification of clones containing putative resistance genes[J].Theoretical and Applied Genetics,2000,101(7):1093-1099.
[19] 潘腾飞,潘东明,姜翠翠,等.琯溪蜜柚 BAC 文库的构建和汁胞粒化相关基因的筛选[J].中国农业科学,2010,43(18):3791-3797.
[20] 王文生,王省芬,马峙英,等.棉花抗黄萎病相关基因筛选与亚克隆文库构建[J].华北农学报,2006,21(Z1):147-150.
[21] 董莉,任雪松,李成琼,等.甘蓝硫甙组分和含量分析[J].西南大学学报:自然科学版,2012,34(12):34-38.
[22] Vicente J G,King G J.Characterization of disease resistance gene-like sequences in Brassica oleracea L[J].Theoretical and Applied Genetics,2001,02:555-563.
[23] Gao M Q,Li G Y,Potter D,et al.Comparative analysis of a Brassica BAC clone containing several major aliphatic glucosinolate genes with its corresponding Arabidopsis sequence[J].Plant Cell Reports,2006,25(6):592-598.
[24] Gao M Q,Li Q Y,McCombie W R,et al.Comparative analysis of a transposon-rich Brassica oleracea BAC clone with its corresponding sequence in A.thaliana [J].Theoretical and Applied Genetics,2005,111(5):949-955.
[25] Dan Q,Gao M Q,Li G Y,et al.Comparative sequence analysis of Brassica oleracea with similar sequences in B.rapa and Arabidopsis thaliana [J].Plant Cell Reports,2009,28(4):649-661.
[26] Zhang H B,Zhao X P,Ding X L,et al.Preparation of megabase-sized DNA from plant nuclei[J].Plant Journal,1995,7(1):175-184.
[27] Ma Z Y,Song W,Peter J S,et al.Non-gridded library:A new approach for BAC(bacterial artificial chromosome) exploitation in hexaploid wheat(Triticum aestivum)[J].Nucleic Acids Research,2000,28(24):106.
[28] 陈书元,晁金泉,徐有明,等.甘蓝型油菜-新疆野生油菜二体异附加系BAC文库的构建[J].中国油料作物学报,2008,30(2):137-142.
[29] 冯大领,石学萍,杨煜,等.大白菜细菌人工染色体文库的构建及鉴定[J].园艺学报,2011,38(1):151-158.
[30] 陈凡国,姜涛,张学勇.BAC文库构建中的几个技术问题探讨[J].生物技术,2002,12(2):28-29.
[31] Zhang H B.Manual for construction and manipulation of large insert bacterial clone libraries(unpublished) [M].Texas:Texas A & M University,2000.
[32] Peterson D G,Tomkins J P,Frisch D A,et al.Construction of plant bacterial artificial chromosome(BAC) libraries:An illustrated guide[J].Agricultural Research Magzine,2000,5:1-100.
[33] Pu Z J,Shimizu M,Zhang Y J,et al.Genetic mapping of a fusarium wilt resistance gene in Brassica oleracea [J].Molecular Breeding,2012,30(2):809-818.
[34] 朱洪运,颉建明,郁继华,等.结球甘蓝AFLP、SSR、SRAP标记高密度遗传图谱构建[J].华北农学报,2013,28(3):82-87.
[35] Tomita H,Motoki S,Doullah M A,et al.Accumulation of quantitative trait loci conferring broad spectrum clubroot resistance in Brassica oleracea [J].Molecular Breeding,2013,32(4):889-900.
[36] Gao M Q,Li G Y,Yang B,et al.High-density Brassica oleracea linkage map:identification of useful new linkages[J].Theoretical and Applied Genetics,2007,115(2):277-287.

Please choose a citation manager

Content to export