Abstract: In order to explore the resistant disease gene and find out relative mechanisms,the suspensions with 1.0×10⁸ colony forming units/mL was sprayed to the resistant variety C7 of seedlings at four to five leaves,while the other group was sprayed distilled water as usual(Control,CK).Leaves were collected in 1,3,5 d time points after XCC and distilled water.A mixture of an equal amount total RNA from the above two groups as two gene pools.Different expression of the genes was analyzed by SRAP technique.The results showed:using 60 pairs of primers,about 685 cDNA fragments in size between 100~750 bp were amplified,averagely 11 bands per primer combination,there were 24 polymorphic loci in these two pool.They were sequenced and the Blast program was used to find out homology with reported genes.Sequence alignment indicated that 6 out of these 9 TDFS(Transcript derived fragments)showed homologies to certain genes from 43% to 59% in NCBI,while the other three showed no significant homology with reported gene.Based on their homologies,these genes were assumed to NADH dehydrogenase,vegetative cell wall protein,DELTA-VPE and unknown functional proteins.The results indicated that cDNA-SRAP method had several advantages over other systems:simplicity,reasonable throughput rate,highly reproducibility and inexpensiveness,and that the cDNA-SRAP technique was applicable to the analysis of gene different expression.Some fragments of differentially expressed genes associated with cabbage black rot were obtained,and may be used for investigating the molecular mechanism of cabbage black rot.

Key words: Cabbage(Brassica oleracea L.var.capitata), Black rot(Xanthomonas Campestris pv.Campestris), cDNA-SRAP

CLC Number: 

• S635.1

• Q78