Abstract: The aim of these study was to clone the promoter of porcine STAB2 gene and analysis its transcriptional activity. Using the gene cloning, dual luciferase reporter gene system and bioinformatics analysis methods, we cloned a 1 997 bp candidate promoter sequence of STAB2 gene and analysed its sequence characteristics, constructed various recombinant vectors with 5' deletions and analysed its luciferase activity by the dual luciferase reporter gene system. Thus, we identified the core promoter region and key regulatory regions of STAB2 gene. We also analysed the transcription factors and their binding sites in the key regulatory regions of STAB2 gene. The results showed that the candidate promoter regions of STAB2 gene contained four core promoters and one CpG island. The-309--39 bp was the key regulatory regions of STAB2 gene's promoter. There was a positive regulatory element in the-1 045--309 bp and a negative regulatory element in the-1 506--1 045 bp of STAB2 gene's promoter. The key regulatory regions of STAB2 gene contained 72 transcription factor binding sites, and some transcription factors had multiple binding sites in this regions, such as Arnt：：Ahr, ZNF354C, Klf4 and KLF5. We also found that coincident region existed between transcription factor binding sites. This study can provide a theoretical basis for further study of the expression regulation mechanism of STAB2 gene and its function in regulating disease resistance and muscle quality in pigs.

Key words: Pig, STAB2, Promoter, Clone, Transcriptional activity

CLC Number: 

• S828.2