Analysis on Differential Expression of MicroRNAs in Different Types of Xanthoceras sorbifolia

doi:10.7668/hbnxb.2014.05.004

Abstract

Abstract: The aim of this paper is to explore miRNA expression specificities in different flower development stages and tissues in different types of Xanthoceras sorbifolia. Flower buds,stems,and leaves of single and double flower types were taken as materials.High quality small RNAs were isolated from four tissues of the two types.11 candidate miRNAs were chosen from small RNAs data bank of Xanthoceras sorbifolia,and qRT-PCR was used to analyze the miRNAs expression in different flower development stages,stems and leaves. The result showed 11 miRNAs had different expression characters.The expression trends of Xso-M007a-b,miR167a-b and miR169 were down-regulated with the flower buds development in two types;on the contrary Xso-M003 was up-regulated.This demonstrated the sequential expression of miRNA.There was also differential expression among different types and tissues.In both types Xso-M002,Xso-M003,Xso-M007a-b,miR169,miR319 and miR827 showed higher expression in flower buds than in stems and leaves.Expression analysis on different types,tissues and time points proved the miRNA's expression specificities:species-specificity,tissue-specificity and time-specificity.In addition,based on miRNA function and expression difference,it was found that morphological characteristics were closely related to miRNA expression.The paper provided valuable information for exploring the function of miRNA expression variation in forest flower formation and tissues differentiation.

Key words: Xanthoceras sorbifolia, miRNA, Real-time quantitative PCR, Expression analysis

CLC Number:

S722
Q78

AO Yan, ZHAO Lei-lei, JIANG Chang-yu, MA Lü-yi. Analysis on Differential Expression of MicroRNAs in Different Types of Xanthoceras sorbifolia[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2014, 29(5): 16-22. doi: 10.7668/hbnxb.2014.05.004.

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