Analyzing Splice Variants and Expression of the OsHSP40 Gene in Rice

doi:10.7668/hbnxb.20191962

Abstract

Abstract: To identify and analyze the expression of the splicers of OsHSP40 using bioinformatics, RT-PCR, TA cloning and sequencing. Firstly, there were 4 kinds of variable splices of OsHSP40 based on bioinformatics analysis. Specific primers were designed for RT-PCR amplification according to the sequence information of different variable splice variants, and combined with TA cloning, successfully identified four kinds of variable splice variants of OsHSP40. To investigate the expression pattern, total RNA was isolated from different tissues such as root, stem, leaf, panicle and seed of WT. RT-PCR results revealed that the expression patterns of OsHsp40.4 and OsHsp40.1234 were different. Finally, the expression of different splice variants under high salt and high temperature conditions was analyzed.The expressions of OsHsp40.4 and OsHsp40.1234 increased under high salt conditions, but the increase range of OsHsp40.4 was relatively low. In addition, after high temperature treatment, the expression of OsHsp40.1234 showed no significant difference at 2 h, and increased significantly at 4 h treatment, while the expression of OsHsp40.4 increased significantly at 2 h treatment. In conclusion, successfully identified four splice variants of OsHSP40, and found differences in the expression of OsHsp40.4 and OsHsp40.1234 in the process of high salt and high temperature stress. These results lay the foundation for further research on the biological function of this gene.

Key words: Oryza sativa, Heat shock proteins(HSPs), OsHSP40, Alternative splicing, Expression analysis

CLC Number:

S511.03
Q78

YU Jinli, LIU Changai, FU Lin, WANG Xin, OUYANG Jiexiu. Analyzing Splice Variants and Expression of the OsHSP40 Gene in Rice[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2021, 36(3): 1-6. doi: 10.7668/hbnxb.20191962.

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