Identification of miRNAs Associated with Incompatible Interaction Between Cucumis metuliferus and Meloidogyne incognita

doi:10.7668/hbnxb.20192373

Abstract

Abstract: The objective of the present study was to identify the plant-derived MicroRNAs(miRNAs), predict the potential target genes and analyze the expression patterns of miRNAs. Small RNA(sRNA)libraries with compatible and incompatible interactions were constructed, sRNAs libraries were then subjected to Solexa sequencing followed by bioinformatics analysis, and miRNAs related to incompatible interaction between C. metuliferus and M. incognita were screened and identified. The target genes of miRNAs were predicted by PsRNATarget software, and the expression of miRNAs was verified by Real-time quantitative expression(qRT-PCR). The results showed that the number of unique reads obtained from 18 samples ranged from 808 262 to 3 926 308, the highest abundance was observed in 24 nt sRNA in length. The miRNAs accounted for only 0.3% -0.5% of all sRNA, including 565 known miRNAs, belonging to 75 miRNAs families, of which miR156 contained the largest number of family members. The expression patterns of 26 differentially expressed miRNAs in response to M. incognita infection can be divided into four types, of which 10 miRNAs were significantly differentially expressed and were closely related to incompatible interaction between C. metuliferus with M. incognita. The prediction of miRNAs target genes showed that one miRNA could regulate multiple target genes, and the target genes of the same miRNA had high homology. The functions of miRNAs target genes are mainly involved in transcriptional activation, participating in plant growth and development and regulation, and encoding proteins related to signal transduction and cell metabolism. These results laid a foundation for exploring the regulatory mechanism of miRNAs in the incompatible interaction between Cucumis metuliferus and Meloidogyne incognita.

Key words: Cucumis metuliferus, Meloidogyne incognita, Incompatible interaction, miRNAs, Identification

CLC Number:

S432.4

YE Deyou, QI Yonghong, ZHANG Huasheng. Identification of miRNAs Associated with Incompatible Interaction Between Cucumis metuliferus and Meloidogyne incognita[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2021, 36(S1): 333-340. doi: 10.7668/hbnxb.20192373.

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