Abstract: In this study,we used the strains F₁-40(Mating type A)and 01-23(Mating type a)as materials.In order to obtain the full-length and the flanking sequence of MAT genes,the MAT1 gene of mating type A strain and the MAT2 gene of mating type a strain of Setosphaeria turcica were cloned by PCR and genome walking with the candidate gene strategy.The conserved domain database of the full-length genes were analyzed.The flanking sequence of MAT1 and MAT2 genes were comprised by the Blast of genomic database of S.turcica. The results suggested that both genes contain one introns.MAT1 encodes amino acids contain a complete MAT α-domain,the domain as part of the MAT alpha1 family.MAT2 gene encodes amino acids,including a complete MATA HMG-box combining with domain,the domain as part of the DNA binding protein in the HMG-box Ⅰ class members of the family,such member containing a single HMG box,composed of three helix,which located between the 133-202 residues in the form of highly sequence specificity combined with DNA minor groove participating in the process of copy transcription and translation of DNA.Then participate in the process of sexual reproduction.Comparing the flanking sequence of MAT1 and MAT2 genes,we discovered the similarity was higher than 93%.

Key words: Setosphaeria turcica, Mating-type, Gene clone, Bioinformatics analysis

CLC Number: 

• Q78

• S435.131