Cloning and Expression Profile Analysis of FT Homologous Gene CsHd3a in Cannabis

doi:10.7668/hbnxb.20191943

Abstract

Abstract: In order to explore the role of the FT gene in the regulation of flowering by the photoperiod pathway of cannabis, the FT homologous gene cDNA sequence was cloned from the industrial cannabis variety Qingma 1 hao (Q1) using PCR technology and named CsHd3a. The sequence characteristics of the gene using biological information were analyzed, and the Real-time fluorescence quantification (qRT-PCR) technology was used to study its tissue-specific expression pattern. The late-flowering variety Yunma 7 hao (Y7) and the early-flowering variety Q1 were selected for long-day and short-day expression profiling using qRT-PCR technology, and the CsHd3a genes of the two varieties were cloned, and the amino acid sequence difference analysis was carried out. The results showed that the CDS sequence of CsHd3a gene was 543 bp in length, encoded 180 amino acids, and contained the protein domain of the PEBP family. Phylogenetic analysis showed that the CsHd3a protein was closely related to the GhFT1 protein of cotton. Tissue-specific analysis showed that the gene was highly expressed in leaves and almost not expressed in roots and stems. The results of qRT-PCR analysis showed that Q1 showed a rhythmic change under short-day (SD) treatment, and reached the highest at 8:00, while it was almost not expressed under long-day (LD) treatment. Under SD conditions, the CsHd3a gene expression of early-flowering variety Q1 was significantly higher than that of late-maturing variety Y7. In addition, the amino acid sequence of CsHd3a had 5 amino acid differences between Q1 and Y7. Whether the difference was due to the change in the function of its conserved domain PEBP remains to be further studied. In summary, this study obtained the CDS sequence of the CsHd3a gene, and initially explored the spatiotemporal expression patterns of CsHd3a genes in the early and late flowering varieties Q1 and Y7, which laid a research foundation for exploring the mechanism of the flowering photoperiod pathway of cannabis.

Key words: Cannabis, CsHd3a, Gene cloning, Bioinformatics analysis, Expression profile analysis

CLC Number:

Q78
S563.03

LI Zheng, PAN Gen, TAO Jie, HUANG Siqi, TANG Huijuan, DENG Yong, ZHAO Lining, LI Defang. Cloning and Expression Profile Analysis of FT Homologous Gene CsHd3a in Cannabis[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2021, 36(3): 41-49. doi: 10.7668/hbnxb.20191943.

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