Genome-Wide Identification and Expression Analysis of the CBDAS Cannabidiolic Acid Synthase Gene Family in Cannabis sativa L.

doi:10.7668/hbnxb.20192457

Abstract

Abstract: In order to reveal the functions of cannabidiolic acid synthase genes(CBDAS), the CBDAS genes in cannabis were identified using bioinformatics methods, and their physical and chemical characteristics, evolutionary relationship, gene structure, the cis-acting elements and transcript patterns were analyzed.Totally, 5 CBDAS genes were identified, and they distributed only on 2 chromosome and 7 chromosome.Their amino acid sequence size, molecular weight and isoelectric point were 541-545 aa, 61.49-62.41 ku, and 6.90-9.00, respectively.Evolution analysis showed that 33 CBDAS gene family members from plants, animals and microorganism were divided into three subclasses, and most of CsCBDAS belonged to the same subclass.The promoters of all CsCBDAS genes were rich in cis -acting elements related to light response.In addition, tissue-specific expression analysis showed that CsCBDAS1 and CsCBDAS2 preferred expressed in female flower and CsCBDAS4 and CsCBDAS5 preferred expressed in root.Moreover, the CsCBDAS members showed different expression patterns between the high-CBD cultivar and low-CBD cultivar, and the expression level of CsCBDAS1 in a high-CBD cultivar was higher than that in a low-CBD cultivar.Exogenous cadmium treatment could increase the exprssion levels of CsCBDAS4 and CsCBDAS5 with cadmium treatment were significantly higher than those without cadmium treatment.The transcript levels of CsCBDAS1, CsCBDAS2 and CsCBDAS5 exhibited significant difference between in light treatment and in darkness treatment, the transcript levels of CsCBDAS1 in light treatment was significantly lower than that in darkness treatment, but the expression levels of CsCBDAS2 and CsCBDAS5 were higher than those in darkness treatment.The CBDAS may involved in the growth and development of cannabis and cannabinoid synthesis, and this study laid the foundation for the further elucidation of the functions of CBDAS genes in cannabis.

Key words: Cannabis sativa L., CBDAS gene family, Expression analysis, Functional analysis

CLC Number:

S563.03
Q78

PAN Gen, TAO Jie, NIE Rong, ZHOU Bing, HUANG Siqi, CHEN Anguo, LI Jianjun, TANG Huijuan, LI Defang, ZHAO Lining. Genome-Wide Identification and Expression Analysis of the CBDAS Cannabidiolic Acid Synthase Gene Family in Cannabis sativa L.[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2021, 36(S1): 1-7. doi: 10.7668/hbnxb.20192457.

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