Cloning and Expression Analysis of BdDREB1H Gene in Brachypodium distachyon

doi:10.7668/hbnxb.20191755

Abstract

Abstract: Plant DREB transcription factor plays an important role in response to abiotic stress such as drought, low temperature and high salinity. In order to investigate the role of DREB in response to stress, we cloned a BdDREB1H gene and analyzed its sequence by bioinformatics and transcriptional activation. Then qRT-PCR was used to study its expression patterns in three tissues of root, stem and leaf and under four abiotic stress conditions of cold, drought, salt and hydrogen peroxide. The results showed that the full-length reading frame of BdDREB1H transcription factor was 735 bp, encoding 244 amino acids, and its relative molecular weight was 26.34 ku. The theoretical isoelectric point was 5.63. The instability index (Ⅱ) was 64.17, indicating it was an unstable protein. The mean total affinity level was-0.261 and was hydrophilic protein. Multiple sequence alignment and domain analysis indicated that BdDREB1H transcription factor contained a typical AP2 domain, which belonged to the DREB subfamily of AP2/EREBP (APETALA2/ethylene responsive element binding protein) superfamily. Evolutionary analysis showed that it was closely related to the CBF of ryegrass, and their sequence similarity was 64.44%. Tissue expression analysis showed that BdDREB1H gene was expressed in all the three tested tissues of root, stem and leaf and had the highest expression level in stem. The expression analysis of stress treatment showed the transcripts of BdDREB1H transcription factor increased firstly and then decreased with time under cold treatment. Under drought and oxidative stresses, the expression level of BdDREB1H was significantly higher than that of the control after 2 hours, while under salt stress, the expression level of BdDREB1H transcription factor was significantly lower than that of control. These results indicated that BdDREB1H transcription factor may be involved in plant stress response, which would lay a foundation for further exploring the molecular mechanism of stress resistance in Brachypodium distachyon.

Key words: Brachypodium distachyon, BdDREB1H, Transcriptional activation analysis, Abiotic stress, Expression analysis

CLC Number:

S572.01
Q78

HUANG Gang, XIONG Qijie, ZHANG Yingjie, ZHOU Junfei, CHEN Lihong. Cloning and Expression Analysis of BdDREB1H Gene in Brachypodium distachyon[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2021, 36(3): 60-66. doi: 10.7668/hbnxb.20191755.

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