Karyotype,Isozyme and Population Genetic Structure Analysis of Macrobrachium rosenbergii

doi:10.7668/hbnxb.20193764

Abstract

Abstract:

In order to evaluate the germplasm resources of M.rosenbergii and provide reference for breeding guidance,it studied the chromosome karyotypes of M.rosenbergii using PHA,narcissin intramuscular injection and the sperms or ovaries cell direct production.The tissue expression patterns of lactate dehydrogenase(LDH),malate dehydrogenase(MDH),esterase(EST)and alcohol dehydrogenase(ADH)of muscle and gill of M.rosenbergii were analyzed by polyacrylamide gel electrophoresis(PAGE).The results showed that M.rosenbergii was diploid,2N=118=56M+24SM+10ST+28T,and arm number(NF)was 198.No sex chromosome or chromosome with satellite or secondary constriction was found.Four isozymes revealed 22 alleles and 15 genetic loci, and the effective number of alleles (Ne) were 1.47.Four enzymes had 7 polymorphic loci with gene frequencies ranging from 0.150 0 to 0.850 0.The proportion of polymorphic sites(P)was 46.7%.The average expected heterozygosity(He)value was 0.191 7,the average observed heterozygosity(Ho)value was 0.261 1,and the Hardy-Weinberg genetic deviation index(D)value was 0.379 7,indicating that there were both heterozygote excess and loss,but the heterozygote excess was more prominent,indicating that this population had a wide range of genetic variation.The degree of variation was large and the population genetic diversity was high,which could provide good parents for future artificial breeding.

Key words: Macrobrachium rosenbergii, Chromosome, Karyotype, Isoenzyme, Genetic structure

ZHANG Lin, GAN Jinhua, ZHANG Tao, HE Li, ZHOU Jianguang. Karyotype,Isozyme and Population Genetic Structure Analysis of Macrobrachium rosenbergii[J]. Acta Agriculturae Boreali-Sinica, 2023, 38(S1): 429-436. doi: 10.7668/hbnxb.20193764.

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Figures/Tables 8

References 24

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染色体编号 Chromosome No.	长臂/μm Long arm	短臂/μm Short arm	总长度/μm Total length	臂比 Arm ratio	相对长度/% Relative length	着丝粒指数 Centromeric index	类型 Classification
1	1.056	0.914	1.970	1.155	2.607	0.464	M
2	0.600	0.459	1.932	1.307	2.557	0.238	M
3	0.921	0.718	1.679	1.283	2.222	0.428	M
4	0.904	0.719	1.657	1.257	2.193	0.434	M
5	0.912	0.550	1.639	1.658	2.169	0.336	M
6	1.016	0.663	1.621	1.532	2.145	0.409	M
7	0.969	0.689	1.566	1.406	2.072	0.440	M
8	0.761	0.690	1.505	1.103	1.992	0.458	M
9	0.928	0.638	1.498	1.455	1.982	0.426	M
10	0.775	0.620	1.471	1.250	1.947	0.421	M
11	1.109	0.663	1.462	1.673	1.935	0.453	M
12	0.782	0.645	1.451	1.212	1.920	0.445	M
13	0.721	0.616	1.428	1.170	1.890	0.431	M
14	0.669	0.667	1.417	1.003	1.875	0.471	M
15	0.787	0.711	1.396	1.107	1.847	0.509	M
16	0.800	0.616	1.376	1.298	1.821	0.448	M
17	0.730	0.645	1.338	1.132	1.771	0.482	M
18	0.757	0.714	1.336	1.060	1.768	0.534	M
19	0.753	0.510	1.301	1.476	1.722	0.392	M
20	0.765	0.535	1.278	1.430	1.691	0.419	M
21	0.621	0.591	1.263	1.051	1.671	0.478	M
22	0.918	0.587	1.212	1.564	1.604	0.484	M
23	0.662	0.434	1.159	1.525	1.534	0.374	M
24	0.616	0.440	1.096	1.400	1.450	0.401	M
25	0.791	0.487	1.096	1.624	1.450	0.401	M
26	0.700	0.459	1.071	1.525	1.417	0.429	M
27	0.663	0.433	1.059	1.531	1.401	0.409	M
28	0.587	0.484	1.056	1.213	1.398	0.458	M
29	1.224	0.708	2.066	1.729	2.734	0.343	SM
30	1.065	0.555	1.620	1.919	2.144	0.343	SM
31	1.428	0.638	1.595	2.238	2.110	0.400	SM
32	1.031	0.564	1.595	1.828	2.111	0.354	SM
33	0.975	0.480	1.455	2.031	1.926	0.330	SM
34	0.975	0.420	1.395	2.321	1.846	0.301	SM
35	0.840	0.465	1.305	1.806	1.727	0.356	SM
36	0.812	0.485	1.297	1.672	1.713	0.324	SM
37	0.855	0.435	1.290	1.966	1.707	0.337	SM
38	0.840	0.405	1.245	2.074	1.648	0.325	SM
39	0.780	0.345	1.125	2.261	1.489	0.307	SM
40	0.750	0.375	1.125	2.000	1.489	0.333	SM
41	0.885	0.285	1.170	3.105	1.548	0.244	ST
42	0.840	0.255	1.095	3.294	1.449	0.233	ST
43	0.855	0.150	1.005	5.700	1.330	0.149	ST
44	0.765	0.225	0.990	3.400	1.310	0.227	ST
45	0.720	0.180	0.900	4.000	1.191	0.200	ST
46	1.245	0.000	1.245	∞	1.648	0.000	T
47	1.155	0.000	1.155	∞	1.529	0.000	T
48	1.125	0.000	1.125	∞	1.489	0.000	T
49	1.095	0.000	1.095	∞	1.449	0.000	T
50	1.080	0.000	1.080	∞	1.429	0.000	T
51	1.050	0.000	1.050	∞	1.390	0.000	T
52	0.990	0.000	0.990	∞	1.310	0.000	T
53	0.975	0.000	0.975	∞	1.290	0.000	T
54	0.975	0.000	0.975	∞	1.290	0.000	T
55	0.960	0.000	0.960	∞	1.270	0.000	T
56	0.900	0.000	0.900	∞	1.191	0.000	T
57	0.855	0.000	0.855	∞	1.131	0.000	T
58	0.795	0.000	0.795	∞	1.052	0.000	T
59	0.794	0.000	0.794	∞	1.051	0.000	T

染色体编号 Chromosome No.	长臂/μm Long arm	短臂/μm Short arm	总长度/μm Total length	臂比 Arm ratio	相对长度/% Relative length	着丝粒指数 Centromeric index	类型 Classification
1	1.056	0.914	1.970	1.155	2.607	0.464	M
2	0.600	0.459	1.932	1.307	2.557	0.238	M
3	0.921	0.718	1.679	1.283	2.222	0.428	M
4	0.904	0.719	1.657	1.257	2.193	0.434	M
5	0.912	0.550	1.639	1.658	2.169	0.336	M
6	1.016	0.663	1.621	1.532	2.145	0.409	M
7	0.969	0.689	1.566	1.406	2.072	0.440	M
8	0.761	0.690	1.505	1.103	1.992	0.458	M
9	0.928	0.638	1.498	1.455	1.982	0.426	M
10	0.775	0.620	1.471	1.250	1.947	0.421	M
11	1.109	0.663	1.462	1.673	1.935	0.453	M
12	0.782	0.645	1.451	1.212	1.920	0.445	M
13	0.721	0.616	1.428	1.170	1.890	0.431	M
14	0.669	0.667	1.417	1.003	1.875	0.471	M
15	0.787	0.711	1.396	1.107	1.847	0.509	M
16	0.800	0.616	1.376	1.298	1.821	0.448	M
17	0.730	0.645	1.338	1.132	1.771	0.482	M
18	0.757	0.714	1.336	1.060	1.768	0.534	M
19	0.753	0.510	1.301	1.476	1.722	0.392	M
20	0.765	0.535	1.278	1.430	1.691	0.419	M
21	0.621	0.591	1.263	1.051	1.671	0.478	M
22	0.918	0.587	1.212	1.564	1.604	0.484	M
23	0.662	0.434	1.159	1.525	1.534	0.374	M
24	0.616	0.440	1.096	1.400	1.450	0.401	M
25	0.791	0.487	1.096	1.624	1.450	0.401	M
26	0.700	0.459	1.071	1.525	1.417	0.429	M
27	0.663	0.433	1.059	1.531	1.401	0.409	M
28	0.587	0.484	1.056	1.213	1.398	0.458	M
29	1.224	0.708	2.066	1.729	2.734	0.343	SM
30	1.065	0.555	1.620	1.919	2.144	0.343	SM
31	1.428	0.638	1.595	2.238	2.110	0.400	SM
32	1.031	0.564	1.595	1.828	2.111	0.354	SM
33	0.975	0.480	1.455	2.031	1.926	0.330	SM
34	0.975	0.420	1.395	2.321	1.846	0.301	SM
35	0.840	0.465	1.305	1.806	1.727	0.356	SM
36	0.812	0.485	1.297	1.672	1.713	0.324	SM
37	0.855	0.435	1.290	1.966	1.707	0.337	SM
38	0.840	0.405	1.245	2.074	1.648	0.325	SM
39	0.780	0.345	1.125	2.261	1.489	0.307	SM
40	0.750	0.375	1.125	2.000	1.489	0.333	SM
41	0.885	0.285	1.170	3.105	1.548	0.244	ST
42	0.840	0.255	1.095	3.294	1.449	0.233	ST
43	0.855	0.150	1.005	5.700	1.330	0.149	ST
44	0.765	0.225	0.990	3.400	1.310	0.227	ST
45	0.720	0.180	0.900	4.000	1.191	0.200	ST
46	1.245	0.000	1.245	∞	1.648	0.000	T
47	1.155	0.000	1.155	∞	1.529	0.000	T
48	1.125	0.000	1.125	∞	1.489	0.000	T
49	1.095	0.000	1.095	∞	1.449	0.000	T
50	1.080	0.000	1.080	∞	1.429	0.000	T
51	1.050	0.000	1.050	∞	1.390	0.000	T
52	0.990	0.000	0.990	∞	1.310	0.000	T
53	0.975	0.000	0.975	∞	1.290	0.000	T
54	0.975	0.000	0.975	∞	1.290	0.000	T
55	0.960	0.000	0.960	∞	1.270	0.000	T
56	0.900	0.000	0.900	∞	1.191	0.000	T
57	0.855	0.000	0.855	∞	1.131	0.000	T
58	0.795	0.000	0.795	∞	1.052	0.000	T
59	0.794	0.000	0.794	∞	1.051	0.000	T

同工酶 Isozyme	基因位点 Genetic locus	等位基因 Allele	基因频率 Gene frequcy	期望杂合度(He) Expected heterozygosity	观测杂合度(Ho) Observed heterozygosity
LDH	LDH-1	a	1.000 0	0.000 0	0.000 0
	LDH-2	a	1.000 0	0.000 0	0.000 0
	LDH-3	a	0.500 0	0.500 0	1.000 0
		b	0.500 0
	LDH-4	a	1.000 0	0.000 0	0.000 0
	LDH-5	a	1.000 0
EST	EST-1	a	0.368 4	0.465 4	0.315 8
		b	0.631 6
	EST-2	a	0.842 1	0.265 9	0.315 8
		b	0.157 9
	EST-3	a	1.000 0	0.000 0	0.000 0
MDH	MDH-1	a	1.000 0	0.000 0	0.000 0
	MDH-2	a	0.357 1	0.459 2	0.526 3
		b	0.642 9
	MDH-3	a	0.578 9	0.487 6	0.842 1
		b	0.421 1
	MDH-4	a	0.666 7	0.444 4	0.666 7
		b	0.333 3
	ADH-1	a	1.000 0	0.000 0	0.000 0
ADH	ADH-2	a	0.150 0	0.255 0	0.300 0
		b	0.850 0
	ADH-3	a	1.000 0	0.000 0	0.000 0
合计Total	15	22		2.875 1	3.966 7
平均值Average				0.191 7	0.261 1

同工酶 Isozyme	基因位点 Genetic locus	等位基因 Allele	基因频率 Gene frequcy	期望杂合度(He) Expected heterozygosity	观测杂合度(Ho) Observed heterozygosity
LDH	LDH-1	a	1.000 0	0.000 0	0.000 0
	LDH-2	a	1.000 0	0.000 0	0.000 0
	LDH-3	a	0.500 0	0.500 0	1.000 0
		b	0.500 0
	LDH-4	a	1.000 0	0.000 0	0.000 0
	LDH-5	a	1.000 0
EST	EST-1	a	0.368 4	0.465 4	0.315 8
		b	0.631 6
	EST-2	a	0.842 1	0.265 9	0.315 8
		b	0.157 9
	EST-3	a	1.000 0	0.000 0	0.000 0
MDH	MDH-1	a	1.000 0	0.000 0	0.000 0
	MDH-2	a	0.357 1	0.459 2	0.526 3
		b	0.642 9
	MDH-3	a	0.578 9	0.487 6	0.842 1
		b	0.421 1
	MDH-4	a	0.666 7	0.444 4	0.666 7
		b	0.333 3
	ADH-1	a	1.000 0	0.000 0	0.000 0
ADH	ADH-2	a	0.150 0	0.255 0	0.300 0
		b	0.850 0
	ADH-3	a	1.000 0	0.000 0	0.000 0
合计Total	15	22		2.875 1	3.966 7
平均值Average				0.191 7	0.261 1

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