Genetic Diversity and Genetic Structure Analysis of Phytophthora nicotianae in Henan by RAPD

doi:10.7668/hbnxb.20190220

Abstract

Abstract: To determine the genetic diversity and genetic structure of Phytophthora nicotianae from Henan Province,34 P. nicotianae isolates were analyzed by randomly amplified polymorphic DNA (RAPD) marker. The results showed that 30 bands were amplified using 6 RAPD primers and 90% of them were polymorphic. Nei's gene diversity index was 0.271 0,and Shannon's information index was 0.402 8. The genetic similarity coefficient of 34 isolates ranged from 0.59 to 1.00. Based on UPGMA dendrogram,34 isolates were divided into 6 groups with 0.80 genetic similarity. Group Ⅰ was the dominant group including 26 isolates. The result of principle coordinate analysis was basically similar to that of UPGMA dendrogram. The result of genetic structure analysis revealed that P.nicotianae population in Henan originated from 2 ancestral subpopulations. The subpopulation Ⅰ and subpopulation Ⅱ accounted for 72.73%,27.27%,respectively. The subpopulation Ⅰ was the dominant subpopulation. Genetic component of 55.88% of isolates almost consisted of only one subpopulation. Therefore,the genetic diversity of P.nicotianae from Henan was low and the genetic structure of them was also simple.

Key words: Phytophthora nicotianae, RAPD, Genetic diversity, Genetic structure

CLC Number:

S432.1

FU Jing, CUI Linkai, FU Wei, QIAO Lijuan. Genetic Diversity and Genetic Structure Analysis of Phytophthora nicotianae in Henan by RAPD[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2020, 35(S1): 342-346. doi: 10.7668/hbnxb.20190220.

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