Species and Pathogenicity Identification of Streptomyces Species Causing Potato Common Scab in Gaomi City, Shandong Province

doi:10.7668/hbnxb.20191183

Abstract

Abstract: To identify and characterize the species of Streptomyces causing potato common scab in Shandong Gaomi. The typical scabby tubers were collected from potato growing areas in Shandong Gaomi from 2018 to 2019. After isolating and purifying,178 Streptomyces strains were obtained from scabby tubers. The pathogenicity of the isolates was determined on tuber slices,radish seedlings method and Koch's rule in greenhouse. A total of 178 strains were screened as pathogenic strains and could induce typical scab symptoms on immature potato. In addition,the potato scab strains isolated in 5 areas of Gaomi City were subjected to morphological identification,PCR molecular identification and pathogenic gene detection. These pathogenic strains were identified and phylogenetic tree was constructed on the basis of the differences in 16S rDNA sequences. Results revealed four kinds of Streptomyces species were found in Gaomi City,Shandong Province,including Streptomyces acidiscabies, Streptomyces scabies, Streptomyces luridiscabiei and Streptomyces griseoaurantiacus, which were consistent with the cluster analysis results. Among them, Streptomyces acidiscabies are dominant species and Streptomyces griseoaurantiacus are newly discovered common scab pathogens in China. The results suggestd that the Streptomyces species causing potato scab had obvious composition diversity and distribution complexity in Shandong Gaomi.

Key words: Potato common scab, Streptomyces, Classification, Pathogenic identification, Diversity

CLC Number:

S432.4

CHEN Lida, LI Lei, XIE Xuewen, SHI Yanxia, CHAI Ali, LI Baoju. Species and Pathogenicity Identification of Streptomyces Species Causing Potato Common Scab in Gaomi City, Shandong Province[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2020, 35(S1): 347-354. doi: 10.7668/hbnxb.20191183.

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