Study on Isolation and Identification of Bacillus thuringiensis and the Effect of Parasporal Crystal Protein Against Lasioderma serricorne

doi:10.7668/hbnxb.20191248

Abstract

Abstract: To discovery of the biological virulence activity of Bacillus thuringiensis spore crystal protein on the Lasioderma serricorne. Using Yunyan No.87 as the test material,the bacterial strain was isolated from the tobacco leaf surface by sodium acetate + antibiotics,and the Bacillus thuringiensis was identified by comparison of physiological and biochemical indexes and 16S rDNA sequencing.The morphology and genotype of its companion parasporal crystal protein were determined,the growth period of Bt strain was investigated,the parasporal crystal protein was isolated,and the toxicity activity of the Lasioderma serricorne was analyzed.The results showed that:the isolated Bt strain could produce the rhomboid cry1 type and the spherical cry8 type parasporal crystal protein,and the parasporal crystal protein had a high toxic activity to the 3-4 week old Lasioderma serricorne.After the treatment with different concentrations of parasporal crystal protein,the Lasioderma serricorne wsa infected after 1 days,the Lasioderma serricorne died and the morphology turned to black and brown. The biological virulence respectively increased with the increase of the infection time and protein concentration gradient.The mortality rates of 150,10 mg/L after 4 days of infection were(96.67±4.22)% and (51.11±5.02)%. These results not only indicated that Bacillus thuringiensis spore crystal protein had a strong biological toxicity to the Lasioderma serricorne,but also provided a technical reference for the further use of biological technology to control tobacco storage pests.

Key words: Nicotiana tobacum, Bacillus thuringiensis, Lasioderma serricorne, Separation identification, Parasporal crystal protein, Virulence activity

CLC Number:

S432.4

LIU Chen, WANG Zhen, ZHU Xianyue, YANG Rui, NI Boli. Study on Isolation and Identification of Bacillus thuringiensis and the Effect of Parasporal Crystal Protein Against Lasioderma serricorne[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2020, 35(S1): 355-362. doi: 10.7668/hbnxb.20191248.

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