Acquirement and Molecular Identification of Transgenic Insect-resistant Corn Expressing Cry1Ac-w Gene

doi:10.7668/hbnxb.2015.05.007

Abstract

Abstract: The purpose of this study was to introduce the modified Cry1Ac-w gene into the corn.Eighty-two regeneration plants were obtained through Agrobacterium -mediated method using embryo callus of the hybrid combination PA×PB as explants and the bialaphos as screening agent,but nineteen plants were putative transgenic plants,confirmed by PCR method of labelled gene(bar)and target gene(Cry1Ac-w).Southern hybridization test(single restriction enzymes digestion)showed successful integration of the foreign Cry1Ac-w gene into the corn genome and the w₁ and w₂ contained one copy and two copies of target gene,respectively.The expressing of Cry1Ac-w protein was confirmed by Bt-Cry1Ab/Ac immune detection strips.

Key words: Corn, Agrobacterium-mediated method, Bacillus thuringiensis (Bt), Cry1Ac-w gene, Southern blot, Immune detection

CLC Number:

YAN Shu-feng, TIE Shuang-gui, YUE Run-qing, HAN Xiao-hua, QI Jian-shuang, LU Cai-xia. Acquirement and Molecular Identification of Transgenic Insect-resistant Corn Expressing Cry1Ac-w Gene[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2015, 30(5): 41-44. doi: 10.7668/hbnxb.2015.05.007.

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http://www.hbnxb.net/EN/Y2015/V30/I5/41

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