Cloning,Expressing Patterns and Constructing Yeast Expression Vectors of Flavanone-3-hydroxylase3(RtF3H3)from Reaumuria trigyna

doi:10.7668/hbnxb.2015.05.008

Abstract

Abstract: Reaumuria trigyna is a small shrub endemic to the Eastern Alxa-Western Ordos area in Inner Mongolia. R.trigyna has a vital ecological function due to its remarkable capability of salt and drought tolerance.Additionally, R.trigyna had been used as traditional herb for the treatment of eczema,dermatitis and other diseases.Flavanone-3-hydroxylase(F3H)is an essential and key enzyme in the flavonoid biosynthetic pathway,it catalyzes the 3-hydroxylation of flavanones.An open reading frame(ORF)of RtF3H3 has been isolated which encodes a protein with 338 amino acids.The theoretical isoelectric point(pI)of RtF3H3 was predicted to be 5.7 and the putative molecular weights was predicted to be 38.46 kDa.The analysis of expressions level of RtF3H3 by quantitative RT-PCR showed that the high transcript levels in stems but low transcript levels in roots and leaves,and the expression level of RtF3H3 was induced by NaCl concentrations and ultraviolet(UV)treatments.In additional,constructing yeast expression vectors of RtF3H3 to lay a foundation for further study of RtF3H3.

Key words: Reaumuria trigyna, F3H gene, Quantitative RT-PCR, Expression vector

CLC Number:

Q943.2

ZHANG Hui-rong, ZHAO Ping-ping, FENG Jie, WANG Ying-chun, ZHENG Lin-lin. Cloning,Expressing Patterns and Constructing Yeast Expression Vectors of Flavanone-3-hydroxylase3(RtF3H3)from Reaumuria trigyna[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2015, 30(5): 45-50. doi: 10.7668/hbnxb.2015.05.008.

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