Abstract: In order to study the B type PI gene of "ABC" model, and explore the gene characteristics of petals and stamen formation.In the study,using CTAB approach to extract the total RNA of the petal of Sedirea japonica,and cloning the part-length cDNA sequences of PI gene of Sedirea japonica by using RT-PCR approach.It found that the length of this gene sequence was 633 bp,it encoded 210 putative amino acid residues and the gene sequence had a high homology with Phalaenopsis equestris after sequence alignment.After analysis of the structure and the hydrophilic and the secondary structure of PI protein,it found that the gene sequence contained the MADS-box domain and K-box,it belonged to the MADS-box super family,PI was hydrophilic protein,and it contained 56.19% Alpha helix,13.81% extended strand and 30% random coil.The expression was analysed by Real-time fluorescent quantitative PCR,PI genes mainly expressed in floral organ,and high expression and PI gene expression had tissue specificity.During the construction of expression vector,it leads the sequence containing objective gene and promoter to the pCAMBIA1301 expression vector,it showed that the construction of expression vector 1301-PI was succeed,which laied the fundation for getting new varieties of Sedirea japonica.

Key words: Sedirea japonica, PI gene, Cloning, Expression analysis, Expression vector

CLC Number: 

• Q949.71+8.43

• Q78