Primary Study on Polymorphism of Wheat Leaf Rust Based IGS

doi:10.7668/hbnxb.2015.05.009

Abstract

Abstract: Wheat leaf rust,caused by Puccinia triticina,is one of the most important and widespread diseases in China.It is necessary to study the virulence and genetic relationship of population structure of wheat leaf rust.IGS(Intergenic spacer)exists between the 28S and 5S transcription region,can be used to identify rust diseases interspecific and intraspecific relationships with different virulences and from different sources.In this study,the use of special primers to amplify Pt's IGS region,found that IGS polymorphism exists between Pt races.And the polymorphism had no direct relationships with the races toxicity,but could concern the areas they came from.IGS-1,IGS-2 were obtained from the two band type by sequencing.The IGS polymorphism could be useful for developing and establishing IGS genetic markers and analyzing the population structure,tracking the origin of annual epidemics and providing insights into evolutionary biology of this pathogen,and planting resistant wheat cultivars to defense the epidemiology of rust.

Key words: Wheat leaf rust, IGS(Intergenic spacer)polymorphism, Regional, Virulence

CLC Number:

S435.121

DU Dong-dong, ZHANG Yu-mei, ZHANG He-shan, YANG Wen-xiang, LIU Da-qun. Primary Study on Polymorphism of Wheat Leaf Rust Based IGS[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2015, 30(5): 51-56. doi: 10.7668/hbnxb.2015.05.009.

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