Resistance Identification of Near-isogenic Lines to Bacterial Blight in Rice Reveals the Multidirectional Effect of Gene Pyramiding

doi:10.7668/hbnxb.20190483

Abstract

Abstract: Gene pyramiding is an effective strategy for breeding broad-spectrum and persistent resistance rice varieties. However, the combination of rice bacterial blight(BB) resistance gene xa5 with some BB resistance genes produced an antagonistic effect. In order to avoid ineffective or antagonistic combinations, it is necessary to evaluate the resistance of rice varieties carrying single gene and gene combinations. We investigated two susceptible rice lines(Indica rice IR24 and Japonica rice TP309), four single-gene resistant lines IRBB3, IRBB5, IRBB21 and CBB23(containing Xa3, xa5, Xa21 and Xa23, respectively), and three gene pyramiding lines IRBB54, IRBB50 and IRBB59(containing xa5 + Xa21, xa5 + Xa4 and xa5 + Xa21 + xa13, respectively) to eight BB strains. The previous reports on the resistance of xa5 and Xa21 single genes showed that the P8 strain overcame the resistance mediated by Xa21.In this study, we found P8 induced the longest lesion of eight strains on both TP309 and IRBB3, which further confirmed the variation of pathogenicity of P8; IRBB54, IRBB50 and IRBB59 showed resistance to all eight strains, indicating the effectiveness of gene pyramiding strategy in dealing with virulence variation of pathogen. The resistance of Xa23 gene to all strains reached the level of IRBB59,but it was weakened after pyramided with xa5,showing a negative antagonistic effect. The results suggested that the pathogenicity variation of stains and the multidirectional effect of gene pyramiding should be fully considered in rice breeding.

Key words: Bacterial blight of rice, Xanthomonas oryzae pv. oryzae, Virulence variation, Gene pyramiding

CLC Number:

Q78
S511.03

ZHOU Junfei, ZHANG Shan, SUN Jing, ZHANG Wei, GAO Lifen. Resistance Identification of Near-isogenic Lines to Bacterial Blight in Rice Reveals the Multidirectional Effect of Gene Pyramiding[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2020, 35(6): 67-73. doi: 10.7668/hbnxb.20190483.

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