Study of Polymerization of Three Disease-resistant Genes by Molecular Marker-assisted Method

doi:10.7668/hbnxb.2015.01.015

Abstract

Abstract: In order to breed new disease-resistant varieties and ensure stable and high-yields of rice, the study on 378 anther culture descendants produced by conventional and anther culture technology was carried out using molecular marker-assisted method.The results showed that three resistance genes of Xa23, Pi-1 and Stvb-i could be polymerized quickly by molecular marker-assisted method.Though the PCR detection and identification of disease resistance, 10 new seeds with stabilized hereditary and high level expression were obtained.The use of molecular marker assisted selection of genotypes at the same time, still needed to phenotype and artificial identification of resistance of the traditional combination, so as to ensure the high accuracy of selected.

Key words: Rice, Marker-assisted selection, Anther culture, Gene pyramiding, Resistance

CLC Number:

S511

SUN Hai-bo, ZOU Mei-zhi, REN Hong-yan, WANG Jing-yu, YAN Shuang-yong, LI Yan-ping, FENG Rui-guang. Study of Polymerization of Three Disease-resistant Genes by Molecular Marker-assisted Method[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2015, 30(1): 90-96. doi: 10.7668/hbnxb.2015.01.015.

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http://www.hbnxb.net/EN/Y2015/V30/I1/90

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