Cloning and Expression Analysis of Ubiquitin-conjugating Enzyme 11(BrUBC11)in Brassica rapa L.subsp. Rapa Tsuda

doi:10.7668/hbnxb.2015.01.016

Abstract

Abstract: In order to elucidate the expression feature of UBC11 in Tsuda, cDNA of UBC11 gene was isolated from this plant.This gene was named BrUBC11 (GenBank accession No.KM396887). BrUBC1I was 685 bp in full length cDNA and 447 bp in full length open reading frame (ORF) encoding 148 amino acids.BrUBC11-GFP was localized to nucleus, indicating that BrUBC11 may play an important role in the nucleus.Quantitative-PCR analysis showed that the BrUBC11 was the highest expressed in the petal and less in bud.The expression of BrUBC11 in Tsuda exhibited tissue specificity.The expression of the BrUBC11 was induced by UV-A light in the swollen hypocotyls.These results indicated of that BrUBC11 had the potential to play a role in response to floral development and UV-A signal transduction pathways, was a multifunction gene.

Key words: Brassica rapa, UBC11, Gene cloning, Expressing analysis

CLC Number:

JIANG Zhong-rong, SHEN Fei, LIU Zhi, GUO Rui, LI Qiao, YAN Hai-fang. Cloning and Expression Analysis of Ubiquitin-conjugating Enzyme 11(BrUBC11)in Brassica rapa L.subsp. Rapa Tsuda[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2015, 30(1): 97-102. doi: 10.7668/hbnxb.2015.01.016.

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http://www.hbnxb.net/EN/Y2015/V30/I1/97

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