Abstract: In this study, based on the full cDNA sequence of PsAP1, the expression pattern and function were analyzed in order to offer theoretical basis about the regulatory mechanism during floral organ development and flowering time and provide candidate genes for breeding of early blooming.Based on the partial sequence in tree Peony obtained by 454 high-throughput sequencing, 1 130 bp PsAP1 full-length cDNA sequence was obtained using RACE amplification.The result of homology analysis indicated that similarity between the PsAP1 protein and VvAP1 was highest with 80.4%.We analyzed its expression pattern in seven different tissues at the early stage of flowering by using Real-time quantitative PCR, and the results showed that the expression level of PsAP1 in sepal was the highest, followed by in petal, the lowest was in stamen.Heterologous expression of the PsAP1 gene in Arabidopsis, and the phenotype of three transgenic lines were identified.The results of phenotypes showed that PsAP1 gene had accelerated early flowering for about 3 days.

Key words: PsAP1, Expression analysis, Heterologous expression, Flowering time

CLC Number: 

• Q78

• S685.03