Cloning and Expression Analysis of a NAC Transcription Factor SlNAC 80 in Tomato

doi:10.7668/hbnxb.2015.01.013

Abstract

Abstract: To further understand the molecular characterization and expression patterns of SlNAC80, we cloned this gene from tomato SlNAC 80 contains an open reading frame of 1 023 bp encoding a protein of 340 amino acids.The molecular weight and isoelectric point of SlNAC80 protein was 38.19 kDa and 5.27, respectively.SlNAC80 contains a typical NAC domain in the N-terminal, which consists of five subdomains (A to E).Quantitative Real-time PCR (qPCR) analysis showed that SlNAC80 was expressed in all tested tomato tissues, with the highest in green fruits and old leaves, and the lowest in red ripe fruits.Promoter sequence analysis indicated that there were many cis-acting regulatory elements involved in response to hormones (ABA, GA, SA and ETH) and abiotic stresses (cold, dehydration and salt stresses), such as ERELEE4, GARE1OSREP1, LTRE1HVBLT49, GT1GMSCAM4, MYB1AT, MYCATERD1, MYCATRD22, WBOXNTERF3, WRKY71OS, and etc.The qPCR analysis also demonstrated that the expression of SlNAC80 was induced by cold, drought, salt, methyl viologen, ABA and ethephon treatments.These results suggest that SlNAC80 may play important roles in regulating abiotic stress responses in tomato.

Key words: Tomato, NAC transcription factor, Gene cloning, Expression analysis, Abiotic stress

CLC Number:

Q78
S641.2

LIU Hui, WANG Tao-tao, ZHANG Jun-hong, OUYANG Bo, LI Han-xia. Cloning and Expression Analysis of a NAC Transcription Factor SlNAC 80 in Tomato[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2015, 30(1): 77-83. doi: 10.7668/hbnxb.2015.01.013.

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References

[1] Puranik S,Sahu P P,Srivastava P S.NAC proteins:regulation and role in stress tolerance[J].Trends in Plant Science,2012,17(6):369-381.
[2] Dung T L,Nishiyama R,Watanabe Y,et al.Genome-wide survey and expression analysis of the plant-specific NAC transcription factor family in soybean during development and dehydration stress[J].DNA Research,2011,18(4):263-276.
[3] Nuruzzaman M,Manimekalai R,Sharoni A M,et al.Genome-wide analysis of NAC transcription factor family in rice[J].Gene,2010,465(1-2):30-44.
[4] Hu R B,Qi G,Kong Y Z,et al.Comprehensive analysis of NAC domain transcription factor gene family in populus trichocarpa[J].BMC Plant Biology,2010,10:145.
[5] Cen ci A,Guignon V,Roux N,et al.Genomic analysis of NAC transcription factors in banana (Musa acuminata) and definition of NAC orthologous groups for monocots and dicots[J].Plant Molecular Biology,2014,85(1-2):63-80.
[6] 杨晓娜,田云,卢向阳.NAC转录因子在植物生长发育中的调控作用[J].化学与生物工程,2014,31(1):1-5,25.
[7] Li W,Huang G Q,Zhou W,et al.A cotton (Gossypium hirsutum) gene encoding a NAC transcription factor is involved in negative regulation of plant xylem development[J].Plant Physiology and Biochemistry,2014,83C:134-141.
[8] Xu Z Y,Kim S Y,Hyeon D Y,et al.The Arabidopsis NAC transcription factor ANAC096 cooperates with bZIP-Type transcription factors in dehydration and osmotic stress responses[J].Plant Cell,2013,25(11):4708-4724.
[9] Liu G Z,Li X E,Jin S X,et al.Overexpression of rice NAC gene SNAC1 improves drought and salt tolerance by enhancing root development and reducing transpiration rate in transgenic cotton[J].PLoS ONE,2014,9(1):e86895.
[10] Chen X,Wang Y F,Lv B,et al.The NAC family transcription factor OsNAP confers abiotic stress response through the ABA pathway[J].Plant and Cell Physiology,2014,55(3):604-619.
[11] 左衍秋,马娜娜,梁晓庆,等.过表达 LeNLP4 转录因子提高番茄抗低温胁迫能力[J].植物生理学报,2014,50(4):501-509.
[12] Dai F W,Zhang C Q,Jiang X Q, et al. RhNAC2 and RhEXPA4 are involved in the regulation of dehydration tolerance during the expansion of rose petals[J].Plant Physiology,2012,160(4):2064-2082.
[13] Jiang X Q,Zhang C Q,Lu P T,et al.RhNAC3,a stress-associated NAC transcription factor,has a role in dehydration tolerance through regulating osmotic stress-related genes in rose petals[J].Plant Biotechnology Journal,2014,12(1):38-48.
[14] Lee S,Seo P J,Lee H J,et al.A NAC transcription factor NTL4 promotes reactive oxygen species production during drought-induced leaf senescence in Arabidopsis[J].Plant Journal,2012,70(5):831-844.
[15] Liu B,Ouyang Z,Zhang Y F, et al. Tomato NAC transcription factor SlSRN1 positively regulates defense response against biotic stress but negatively regulates abiotic stress response[J].PLoS ONE,2014,9(7):e102067.
[16] 陈秀玲,王傲雪,张珍珠,等.番茄NAC转录因子家族的鉴定及生物信息学分析[J].植物生理学报,2014,50(4):461-470.
[17] Liu H,Ouyang B,Zhang J H,et al.Differential modulation of photosynthesis,signaling,and transcriptional regulation between tolerant and sensitive tomato genotypes under cold stress[J].PLoS ONE,2012,7(11):e50785.
[18] Lovdal T,Lillo C.Reference gene selection for quantitative real-time PCR normalization in tomato subjected to nitrogen,cold,and light stress[J].Analytical Biochemistry,2009,387(2):238-242.
[19] Livak K J,Schmittgen T D.Analysis of relative gene expression data using real-time quantitative PCR and the 2^-ΔΔCT method [J].Methods,2001,25(4):402-408.
[20] Higo K,Ugawa Y,Iwamoto M,et al.Plant cis-acting regulatory DNA elements (PLACE) database:1999[J].Nucleic Acids Research,1999,27(1):297-300.
[21] 邢国芳,张雁明,张魏斌,等.植物NAC转录因子的研究进展[J].山西农业科学,2012,40(4):409-411,423.
[22] 张进艳,陈芳,李亮,等.水分胁迫下16个玉米NAC转录因子的序列特征和表达分析[J].山西农业科学,2014,42(4):307-312
[23] 李伟,韩蕾,钱永强,等.植物NAC转录因子的种类、特征及功能[J].应用与环境生物学报,2011,17(4):596-606.
[24] Zhou Y,Huang W F,Liu L,et al.Identification and functional characterization of a rice NAC gene involved in the regulation of leaf senescence[J].BMC Plant Biology,2013,13:132.

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