Abstract: In order to breeding disease-resistant rice varieties to ensure stable and high-yields of rice, the breeding of disease-resistant germplasm was studied by methods of conventional, anther culture and molecular marker-assisted technology.The results showed that, after the gene Stvb-i resistant to stripe disease was hybridized by BG152 of bacterial leaf blight resistant gene Xa23 and R118 of rice blast resistant gene Pi-1 respectively, 180 and 105 the seeds F₀ with double resistant disease gene were obtained.Those seeds were re-crossed after identification of the true ones and 1 398 F₀ hybrids were screened.52 single plants with disease resistant to three genes were obtained by molecular marker-assisted technology.Furthermore, 378 plants with double anther-culture were gotten based on the selection and culture of no disease samples in field after H₀ natural doubling.14 strains of anther culture with three disease-resistant genes were obtained though the PCR detection.After multiple identification of PCR and disease resistance of their H₂ plants, 10 new seeds with stabilized hereditary and high level expression were obtained.At last, the analysis of the problem during the course of rice anther culture was carried out and the prevention and corresponding actions were proposed also.

Key words: Rice, Marker-assisted selection, Anther culture, Gene pyramiding, Resistance

CLC Number: 

• S511.03

• S435