Identification of Wheat Leaf Rust Resistance Gene in Henong 6251 at Seedling Stage

doi:10.7668/hbnxb.2015.03.033

Abstract

Abstract: Preliminary research showed that they all contributed higher disease resistance level to Puccinia triticina.Detecting and mapping wheat leaf rust resistance gene were significance for application of the resistance in the wheat production.Resistant parents Henong 6251,susceptible parent Thatcher and their F₂,F₃ populations were employed to map the wheat leaf rust resistance genes in Henong 6251 based on SSR technique.Molecular marker-assisted identification results showed that Henong 6251 contained wheat leaf rust resistance genes Lr26 and LrZH84,and probably contained Lr2c and Lr17a.Henong 6251,Thatcher,and their F_l,F₂ populations and F₃ families were tested for detecting the wheat leaf rust resistance with single strain of Chinese P.triticina pathotype PBGP in greenhouse.The results showed that the wheat leaf rust resistance in Henong 6251 to PBGP was controlled by one dominant resistance gene,temporarily designated as LrH6.SSR molecular markers and BSA were used to test Henong 6251 and their resistant and susceptible bulks of F₂ and F₃populations for mapping the leaf rust resistance gene.Results indicated that Henong 6251 carried a single dominant resistance gene LrH6 to PBGP,and was preliminary located on chromosome 1B.The resistance gene LrH6 was linked to the three known SSR loci(wmc419,wms582 and barc120)on the 1BL with genetic distance ranging 21.6,25.8,27.9 cM respectively.Henong 6251 contained more than one leaf rust resistance genes including Lr26 and LrZH84 and the wheat leaf rust resistance to PBGP was controlled by one dominant and new resistance gene.

Key words: Wheat leaf rust, Resistance gene, SSR marker, Genetic analysis, Genetic mapping

CLC Number:

S435.121

REN Xiao-di, DU Dong-dong, ZHANG He-shan, YAO Hong-peng, ZHAO Sai, YANG Wen-xiang, LIU Da-qun. Identification of Wheat Leaf Rust Resistance Gene in Henong 6251 at Seedling Stage[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2015, 30(3): 192-199. doi: 10.7668/hbnxb.2015.03.033.

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