Abstract: Different DNA isolation methods were used in researches due to the diversity of secondary metabolites in different plant tissues.The existing of large amounts of polysaccharides,proteins,polyphenols and pigments in strawberry plants influenced the extraction of high quality DNA from strawberry leaves and afterwards PCR reaction.In order to identify the DNA extraction method which was applied to the PCR detection for Strawberry vein banding virus (SVBV),total DNAs extracted from strawberry leaves by three different extraction methods to detect SVBV by PCR were tested and compared in this study.Strawberry leaves infected by SVBV were used as the materials from which CTAB kit method,high salt and low pH method (HSLPM) and modified SDS method were used to extract DNA in this paper.The quality and quantity of DNA extracted by these three different methods were analyzed by gel electrophoresis and UV spectrophotometers.SVBV was detected by PCR from extracted DNA to determine the effect of different DNA extraction methods on the stability of PCR detection.The results showed that all the three methods could isolate complete DNA fragments.Sequencing and sequence analysis of the PCR products from strawberry leaves infected by SVBV proved the reliability of the methods.DNA extracted by three methods from strawberry leaves could be detected the infection of SVBV showed the quality of DNA extracted by all the three methods could meet the specific demands of PCR detection of SVBV in strawberry leaves.Compared with the other two extraction methods,results showed the quality of DNA extracted by CTAB kit was the best in these three different DNA extraction methods in this paper.

Key words: DNA extraction from plant tissues, CTAB kit, High salt and low pH method, Modified SDS method

CLC Number: 

• S432.4+1