Detecting and Analysis of Rice Black Stripe Dwarf Virus(RBSDV)on Rice in Jiangsu Province with RT-PCR

doi:10.7668/hbnxb.2008.06.020

Abstract

Abstract: To determine the pathogen of rice dwarf disease which has caused yield loss in Jiangsu Province in recent years was caused by rice black stripe dwarf virus(RBSDV).Four pairs of primers to amplify RNA dependent RNA polymerase and the coat protein and core protein and silencing suppressor of RBSDV were designed.Four bands with about 200 bp to 300 bp were obtained in infected rice plants by RTM-PCR,respectively.The separate segment was ligated top GEM-T-easy vector,then sequenced with M13 primers.Their identities to rice black-streaked dwarf virus were higher than 99 % and seven synonymous substitution were found.The results showed that the virus which causes rice dwarf disease in Jiangsu is RBSDV.The identification of RBSDV in the small brown planthopper(Laodelphax striatellus),rice and other host plants is of great importance for the disease forecasting.This approach can be used for early detection of RBS-DV in infected rice and other host plants.

Key words: Rice black stripe dwarf virus disease, RT-PCR, Virus detection

CLC Number:

S432.4+1

YANG Jie, WANG Jun, ZHOU Tong, CHEN Zhi-de, ZHONG Wei-gong. Detecting and Analysis of Rice Black Stripe Dwarf Virus(RBSDV)on Rice in Jiangsu Province with RT-PCR[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2008, 23(6): 87-92. doi: 10.7668/hbnxb.2008.06.020.

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