Cloning and Expression Analysis of Distant Hybridization Incompatibility PlABCF3 Gene from Paeonia

doi:10.7668/hbnxb.20191499

Abstract

Abstract: To further study the mechanism of PlABCF3 gene in distant hybrid incompatibility of Paeonia lactiflora. The stigmas of combinations P. lactiflora Fenyunu×P. lactiflora Fenyunu and P. lactiflora Fenyunu×P. ostii Fengdanbai were harvested as the materials after 24,36 h of pollination and ABCF3 gene(PlABCF3) was cloned by RT-PCR according to transcriptome data. Bioinformatics and expression characteristics showed that the CDS of PlABCF3 was 2 151 bp encoded 716 amino acids. Between PlABCF3 and ABCFs in Arabidopsis thaliana, phylogenetic analysis and homologous sequence analysis showed that there were high homology of base sequences between PlABCF3 and AtABCF3. Phylogenetic analysis showed that PlABCF3 had high identities with ABCF3 in Macleaya cordata, and the amino acid sequence of PlABCF3 had high similarity with ABCF3 in Macleaya cordata,so its name was designated PlABCF3 (GenBank accession No.MT123594). RT-qPCR results indicated that during self-cross and hybridization of the stigmas,the PlABCF3 expression level was lower in 36 h at the same treatment and lowest in hybridization stigma in 36 h. PlABCF3 gene expression was negatively related with abscisic acid(ABA), and zeatin nucleoside(ZR) content in stigmas(R=-0.837,-0.565,P<0.01). It was speculated that PlABCF3 had a deep connection with endogenous hormones in the distant hybridization of Paeonia.

Key words: Paeonia ostii, Paeonia lactiflora, PlABCF3 gene, RT-PCR, Distant hybridization

CLC Number:

S682.1+2

HE Dan, ZHANG Jiaorui, HE Songlin, LIU Hongli, WANG Zheng, LIU Yiping. Cloning and Expression Analysis of Distant Hybridization Incompatibility PlABCF3 Gene from Paeonia[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2020, 35(6): 81-89. doi: 10.7668/hbnxb.20191499.

/ / Recommend / Download Citations

URL: http://www.hbnxb.net/EN/10.7668/hbnxb.20191499

http://www.hbnxb.net/EN/Y2020/V35/I6/81

References

[1] 马翔龙,吴敬需,刘少华.伊藤牡丹发展现状与展望[J].中国花卉园艺, 2018(16):28-31.doi:10.3969/j.issn.1009.8496.2018.16.013.
Ma X L, Wu J X, Liu S H. Present situation and prospect of the development of Itoh hybrid peonies[J]. China Flowers & Horticulture, 2018(16):28-31.
[2] 杨柳慧,张建军,王琪,朱炜,张滕,于晓南.5个芍药属伊藤杂种的倍性鉴定及核型分析[J].植物研究, 2017, 37(4):535-541.doi:10.7525/j.issn.1673-5102.2017.04.008.
Yang L H, Zhang J J, Wang Q, Zhu W, Zhang T, Yu X N. Ploidy identification and karyotype analysis of five Itoh hybrid peonies[J]. Bulletin of Botanical Research, 2017, 37(4):535-541.
[3] Hao Q, Aoki N, Katayama J, Kako T, Cheon K S, Akazawa Y, Kobayashi N. Crossability of American tree peony ‘High Noon’ as seed parent with Japanese cultivars to breed superior cultivars[J]. Euphytica, 2013, 191:35-44.doi:10.1007/s10681-012-0853-3.
[4] Ma K F, Song Y P, Huang Z, Lin L Y, Zhang Z Y, Zhang D Q. The low fertility of Chinese white poplar:dynamic changes in anatomical structure, endogenous hormone concentrations, and key gene expression in the reproduction of a naturally occurring hybrid[J]. Plant Cell Reports, 2013, 32:401-414.doi:10.1007/s00299-012-1373-2.
[5] Mesejo C, Yuste R, Martínez-Fuentes A, Reig C, Iglesias D J, Primo-Millo E, Agust M. Self-pollination and parthenocarpic ability in developing ovaries of self-incompatible clementine mandarins(Citrus clementina)[J]. Physiology Plant, 2013, 148(1):87-96.doi:10.1111/j.1399-3054.2012.01697.x.
[6] 张鹏,周骏辉,荆艳萍.杨树授粉亲和性与雌蕊生理生化变化的关系[J].东北林业大学学报, 2014, 42(6):11-14, 33.doi:10.3969/j.issn.1000-5382.2014.06.003.
Zhang P, Zhou J H, Jing Y P. Relationship between physiological and biochemical changes in pistil and pollination compatibility of popla[J]. Journal of Northeast Forestry University, 2014, 42(6):11-14, 33.
[7] 贺丹,解梦珺,吕博雅,王政,刘艺平,何松林.牡丹与芍药的授粉亲和性表现及其生理机制分析[J].西北农林科技大学学报(自然科学版), 2017, 45(10):129-136.doi:10.13207/j.cnki.jnwafu.2017.10.016.
He D, Xie M J, Lü B Y, Wang Z, Liu Y P, He S L. Analysis of pollination affinity performance and its physiological mechanism in Paeonia sufruticosa and Paeonia factiflora[J]. Journal of Northwest A & F University(Natural Science Edition), 2017, 45(10):129-136.
[8] Chien V H, Leyva-González M A, Osakabe Y, Tran U T, Nishiyama R, Watanabe Y, Tanaka M, Seki M, Yamaguchi S, Dong N V, Yamaguchi-Shinozaki K, Shinozaki K, Herrera-Estrella L, Tran L P. Plant ABC proteins-a unified nomenclature and updated inventory[J]. Trends Plant Science, 2008, 13(4):151-159.doi:10.1016/j.tplants.2008.02.001.
[9] 曹冠华,柏旭,陈迪,张晓蓉,贺森.ABC转运蛋白结构特点及在植物和真菌重金属耐性中的作用与机制[J].农业生物技术学报, 2016, 24(10):1617-1628.doi:10.3969/j.issn.1674-7968.2016.10.019.
Cao G H, Bai X, Chen D, Zhang X R, He S. Structure characteristics of ABC transporter protein and the function and mechanism on enhancing resistance of plants and fungi to heavy metals[J]. Journal of Agricultural Biotechnology, 2016, 24(10):1617-1628.
[10] Ponte-Sucre A. Availability and applications of ATP-binding cassette(ABC) transporter blockers[J]. Applied Microbiology and Biotechnology, 2007, 76(2):279-286.doi:10.1007/s00253-007-1017-6.
[11] Dean M, Rzhetsky A, Allikmets R. The human ATP-binding cassette(ABC) transporter super family[J]. Genome Research, 2001,11(7):1156-1166.doi:10.1101/gr.184901.
[12] Dean M, Annilo T. Evolution of the ATP-binding cassette(ABC) transporter superfamily in vertebrates[J]. Annual Review of Genomics and Human Genetics, 2005, 6:123-142.doi:10.1146/annurev.genom.6.080604.162122.
[13] Kubeš M, Yang H B, Richter G L, Cheng Y, Młodzińska E, Wang X, Blakeslee J J, Carraro N, Petrášek J, Zažímalová E, Hoyerová K, Peer W A, Murphy A S. The Arabidopsis concentration-dependent influx/efflux transporter ABCB4 regulates cellular auxin levels in the root epidermis[J]. The Plant Journal, 2012, 69(4):640-654.doi:10.1111/j.1365-313X.2011.04818.x.
[14] Kaneda M, Schuetz M, Lin B S P, Chanis C, Hamberger B, Western T L, Ehlting J, Samuels A L. ABC transporters coordinately expressed during lignification of Arabidopsis stems include a set of ABCBs associated with auxin transport[J]. Journal of Experimental Botany, 2011, 62(6):2063-2077.doi:10.1093/jxb/erq416.
[15] Kuromori T, Miyaji T, Yabuuchi H, Shimizu H, Sugimoto E, Kamiya A, Moriyama Y, Shinozaki K. ABC transporter AtABCG25 is involved in abscisic acid transport and responses[J]. Proceedings of the National Academy of Sciences of the USA, 2010, 107(5):2361-2366.doi:10.1073/pnas.0912516107.
[16] Merilo E, Jalakas P, Laanemets K, Mohammadi O, Hõrak H, Kollist H, Brosché M. Abscisic acid transport and homeostasis in the context of stomatal regulation[J]. Molecular Plant, 2015, 8(9):1321-1333.doi:10.1016/j.molp.2015.06.006.
[17] Kuromori T, Sugimoto E, Shinozaki K. Arabidopsis mutants of AtABCG22, an ABC transporter gene, increase water transpiration and drought susceptibility[J]. The Plant Journal, 2011, 67(5):885-894.doi:10.1111/j.1365-313X.2011.04641.x.
[18] Chien V H, Leyva-Gonzálezc M A, Osakabe Y, Uyen T T, Rie N, Yasuko W, Maho T, Motoaki S, Shinjiro Y, Nguyen V D, Kazuko Y S, Kazuo S, Luis H E, Lam P T. Positive regulatory role of strigolactone in plant responses to drought and salt stress[J]. Proceedings of the National Academy of Sciences of the USA, 2014, 111(2):851-856.doi:10.1073/pnas.1322135111.
[19] 孟冬.苹果MdABCF转运S-RNase至花粉管影响自交不亲和反应[D].北京:中国农业大学, 2014.
Meng D. Apple ABCF transport S-RNase into pollen tube effecting self-incompatibility[D]. Beijing:China Agriculture University, 2014.
[20] 李依民,雷根平,颜永刚,彭亮, 张娜, 刘亮亮, 黑小斌, 李欢, 张岗, 郭顺星.铁皮石斛2个F家族ABC转运蛋白基因的克隆和表达研究[J].中草药, 2017, 48(15):3153-3159.doi:10.7501/j.issn.0253-2670.2017.15.022.
Li Y M, Lei G P, Yan Y G, Peng L, Zhang N, Liu L L, Hei X B, Li H, Zhang G, Guo S X. Molecular cloning and expression analysis of two genes encoding F family ATP-binding cassette transporters in Dendrobium officinale[J]. Chinese Traditional and Herbal Drugs, 2017, 48(15):3153-3159.
[21] He D, Lou X Y, He S L, Lei Y K, Lü B V, Wang Z, Zheng Y B, Liu Y P. Isobaric tags for relative and absolute quantitation-based quantitative proteomics analysis provides novel insights into the mechanism of cross-incompatibility between tree peony and herbaceous peony[J]. Functional Plant Biology, 2019, 46(5):417-427.doi:10.1071/FP18163.
[22] 贺丹,王雪玲,高晓峰,吕博雅,刘艺平,何松林.牡丹芍药远缘杂交亲和性[J].东北林业大学学报, 2014(7):65-68.doi:10.13759/j.cnki.dlxb.2014.07.016.
He D, Wang X L, Gao X F, Lü B Y, Liu Y P, He S L. Intergeneric cross-compatibility between peonies[J]. Journal of Northeast Forestry University, 2014(7):65-68.
[23] Schmittgen T D, Livak K J. Analyzing Real-time PCR data by the comparative C_T method[J]. Nature Protocols, 2008, 3(6):1101-1108.doi:10.1038/nprot.2008.73.
[24] Wu J Z, Qin Y, Zhao J. Pollen tube growth is affected by exogenous hormones and correlated with hormone changes in styles in Torenia fournieri L.[J]. Plant Growth Regulation, 2008, 55(2):137-148.doi:10.1007/s10725-008-9268-5.
[25] 许明,白明义,魏毓棠.紫菜薹细胞质雄性不育系及其保持系在不同发育时期内源激素的变化[J].西北农业学报, 2007, 16(3):124-127,135.doi:10.3969/j.issn.1004-1389.2007.03.032 Xu M, Bai M Y, Wei Y T. Changes in endogenous hormone between B. campestris ssp. chinensis var. purpurea Hort. CMS linesand their maintainer line at different development stages[J]. Acta Agriculturae Boreali-occidentalis Sinica, 2007, 16(3):124-127,135.
[26] Chen D, Zhao J. Free IAA in stigmas and styles during pollen germination and pollen tube growth of Nicotiana tabacum[J]. Physiologia Plantarum, 2008, 134(1):202-215.doi:10.1111/j.1399-3054.2008.01125.x
[27] Geisler M. Plant ABC transporters[M]. Switzerland:Springer International Publishing, 2014.
[28] Kang J Y, Hwang J U, Lee M, Kim Y Y, Assmann S M, Martinoia E, Lee Y. PDR-type ABC transporter mediates cellular uptake of the phytohormone abscisic acid[J]. Proceedings of the National Academy of Sciences of the United States of America,2010, 107(5):2355-2360.doi:10.1073/pnas.0909222107.
[29] 邵若玄,沈忆珂,周文彬,方佳,郑炳松.植物ATP结合盒(ABC)转运蛋白研究进展[J].浙江农林大学学报, 2013, 30(5):761-768.doi:10.11833/j.issn.2095-0756.2013.05.020.
Shao R X, Shen Y K, Zhou W B, Fang J, Zheng B S. Recent advances for plant ATP-binding cassette transporters[J]. Journal of Zhejiang A&F University, 2013, 30(5):761-768.
[30] Kamimoto Y, Terasaka K, Hamamoto M, Takanashi K, Fukuda S, Shitan N, Sugiyama A, Suzuki H, Shibata D, Wang B J, Pollmann S, Geisler M, Yazaki K. Arabidopsis ABCB21 is a facultative auxin importer/exporter regulated by cytoplasmic auxin concentration[J]. Plant and Cell Physiology, 2012, 53(12):2090-2100.doi:10.1093/pcp/pcs149.
[31] 阎波,刘思思,陈娟,郭顺星.药用植物铁皮石斛ABC转运蛋白基因的鉴定及其差异表达分析[J].药学学报, 2018, 53(7):1177-1189.doi:10.16438/j.0513-4870.2018-0117.
Yan B, Liu S S, Chen J, Guo S X. Identification and differential expression analysis of ABC transporter gene from medicinal plant Dendrobium officinale[J]. Acta Pharmaceutica Sinica, 2018, 53(7):1177-1189.
[32] 吕换男,马聚泽,郝艳,左波,黄佳,王海静,武军凯.‘鸭梨’及其自交亲和性芽变‘金坠梨’花粉ABC转运蛋白家族分析[J].河北科技师范学院学报, 2019, 33(2):1-11.doi:10.3969/J.ISSN.1672-7983.2019.02.001.
Lü H N, Ma J Z, Hao Y, Zuo B, Huang J, Wang H J, Wu J K. Proteomic analysis of ABC transporters from self-incompatible ‘Yali’ and its spontaneous self-compatibl mutant ‘Jinzhuili’[J]. Journal of Hebei Normal University of Science & Technology, 2019, 33(2):1-11.
[33] Meng D, Gu Z Y, Li W, Wang A D, Yuan H, Yang Q, Li T Z. Apple MdABCF assists in the transportation of S-RNase into pollen tubes[J]. The Plant Journal, 2014, 78(6):990-1002.doi:10.1111/tpj.12524.
[34] Goldraij A, Kondo K, Lee C B, Hancock C N, Sivaguru M, Vazquez-Santana S, Kim S, Phillips T E, Cruz-Garcia F, McClure B. Compartmentalization of S-RNase and HT-B degradation in self-incompatible Nicotiana[J]. Nature, 2006, 439(7078):805-810.doi:10.1038/nature04491.
[35] Luu D T, Qin X K, Morse D, Cappadocia M.S-RNase uptake by compatible pollen tubes in gametophytic self-incompatibility[J]. Nature, 2000, 407(6804):649-651.doi:10.1038/35036623.
[36] Choi H, Jin J Y, Choi S, Hwang J U, Kim Y Y, Suh M C, Lee Y. An ABCG/WBC-type ABC transporter is essential for transport of sporopollenin precursors for exine formation in developing pollen[J]. The Plant Journal:for Cell and Molecular Biology, 2011, 65(2):181-193.doi:10.1111/j.1365-313X.2010.04412.x.

Please choose a citation manager

Content to export